Document Detail

Induction of endoreduplication in temperature-sensitive mutants of rat 3Y1 fibroblasts.
MedLine Citation:
PMID:  3968187     Owner:  NLM     Status:  MEDLINE    
Four temperature-sensitive mutants of rat 3Y1 fibroblasts belonging to separate complementation groups (3Y1tsD123, 3Y1tsF121, 3Y1tsG125, and 3Y1tsH203) are arrested mainly with a 2C DNA content, when cells proliferating at 33.8 degrees C are shifted up to 39.8 degrees C (Ohno et al., 1984). Zaitsu and Kimura (submitted for publication) showed that 3Y1tsF121 cells synchronized in the early S phase were arrested with a 4C DNA content at 39.8 degrees C. We studied the traverse through the S and G2 phases at 39.8 degrees C in the four ts mutants synchronized at the early S phase and found that 3Y1tsG125 and 3Y1tsH203 cells were arrested with a 4C DNA content as 3Y1tsF121, while 3Y1tsD123 cells went through S and G2 phases and underwent mitosis. When 3Y1tsF121 and 3Y1tsG125 mutants arrested at 39.8 degrees C were shifted down to 33.8 degrees C, a substantial fraction of the cells with a 4C DNA content started, with a certain lag period, DNA synthesis without intervening mitosis and underwent the first mitosis with a lag period similar to that in the cells arrested with a 2C DNA content. The tetraploid cells thus generated had a proliferating ability lower than that of diploid cells.
K Yamada; G Kimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  122     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-03-06     Completed Date:  1985-03-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  210-4     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Division
DNA / metabolism
Fibroblasts / cytology*,  metabolism
Time Factors
Reg. No./Substance:

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