| Induction of differentiation of U-937 cells by 2-chloro-3-amino-1,4-naphthoquinone. | |
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MedLine Citation:
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PMID: 9344233 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Naphthoquinone compounds have various pharmacological effects such as antiviral, antifungal and anticancer activities. We demonstrated the differentiation of the inducing effect of a naphthoquinone derivative, 2-chloro-3-amino-1,4-nahpthoquinone (NQCA) on the human leukemia cell line U-937. When U-937 cells were treated with NQCA for 4 days, phenotypes indicative of differentiation such as nitroblue tetrazolium (NBT)-reducing activity and phagocytosis were induced. To evaluate the route of differentiation of U-937 cells induced by NQCA, we determined naphthol AS-D chloroacetate esterase and alpha-naphthyl acetate esterase activities. Four days treatment of U-937 cells with NQCA increased alpha-naphthyl acetate esterase activity about 63.5% but naphthol AS-D chloroacetate esterase was not detected. These results indicate that NQCA caused differentiation of U-937 cells into macrophage-like cells. Since protein kinase C (PKC) and protein kinase A (PKA) have important roles in cell-differentiation and proliferation, we employed a PKC inhibitor NA-382 and a PKA inhibitor H-89 to examine the effects of each kinase on the differentiation of U-937 cells. The PKC inhibitor NA-382 decreased the effect of NQCA on U-937 cells, while the PKA inhibitor H-89 did not. Also glutathione (GSH) inhibited the effect of NQCA. It is concluded that the differentiation-inducing effect of NQCA on U-937 cells may be attributed to PKC activation followed by production of free radicals. |
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Authors:
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H Kwon; S Y Choung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Research communications in molecular pathology and pharmacology Volume: 97 ISSN: 1078-0297 ISO Abbreviation: Res. Commun. Mol. Pathol. Pharmacol. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-11-17 Completed Date: 1997-11-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9437512 Medline TA: Res Commun Mol Pathol Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 215-27 Citation Subset: IM |
Affiliation:
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Department of Hygienic Chemistry, College of Pharmacy, Kyoung Hee University, Seoul, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaloids
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pharmacology Cell Differentiation / drug effects* Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors* Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Glutathione / metabolism Humans Isoquinolines / pharmacology Naphthol AS D Esterase / analysis Naphthoquinones / pharmacology* Nitroblue Tetrazolium Phagocytosis / drug effects Protein Kinase C / antagonists & inhibitors* Staurosporine / analogs & derivatives Sulfonamides* Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Alkaloids; 0/Enzyme Inhibitors; 0/Isoquinolines; 0/Naphthoquinones; 0/Sulfonamides; 127243-85-0/H 89; 143086-33-3/N-ethoxycarbonyl-7-oxostaurosporine; 2797-51-5/2-chloro-3-amino-1,4-naphthoquinone; 298-83-9/Nitroblue Tetrazolium; 62996-74-1/Staurosporine; 70-18-8/Glutathione; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.13/Protein Kinase C; EC 3.1.-/Naphthol AS D Esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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