Document Detail


Induction of differentiation of U-937 cells by 2-chloro-3-amino-1,4-naphthoquinone.
MedLine Citation:
PMID:  9344233     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Naphthoquinone compounds have various pharmacological effects such as antiviral, antifungal and anticancer activities. We demonstrated the differentiation of the inducing effect of a naphthoquinone derivative, 2-chloro-3-amino-1,4-nahpthoquinone (NQCA) on the human leukemia cell line U-937. When U-937 cells were treated with NQCA for 4 days, phenotypes indicative of differentiation such as nitroblue tetrazolium (NBT)-reducing activity and phagocytosis were induced. To evaluate the route of differentiation of U-937 cells induced by NQCA, we determined naphthol AS-D chloroacetate esterase and alpha-naphthyl acetate esterase activities. Four days treatment of U-937 cells with NQCA increased alpha-naphthyl acetate esterase activity about 63.5% but naphthol AS-D chloroacetate esterase was not detected. These results indicate that NQCA caused differentiation of U-937 cells into macrophage-like cells. Since protein kinase C (PKC) and protein kinase A (PKA) have important roles in cell-differentiation and proliferation, we employed a PKC inhibitor NA-382 and a PKA inhibitor H-89 to examine the effects of each kinase on the differentiation of U-937 cells. The PKC inhibitor NA-382 decreased the effect of NQCA on U-937 cells, while the PKA inhibitor H-89 did not. Also glutathione (GSH) inhibited the effect of NQCA. It is concluded that the differentiation-inducing effect of NQCA on U-937 cells may be attributed to PKC activation followed by production of free radicals.
Authors:
H Kwon; S Y Choung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research communications in molecular pathology and pharmacology     Volume:  97     ISSN:  1078-0297     ISO Abbreviation:  Res. Commun. Mol. Pathol. Pharmacol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-11-17     Completed Date:  1997-11-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9437512     Medline TA:  Res Commun Mol Pathol Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  215-27     Citation Subset:  IM    
Affiliation:
Department of Hygienic Chemistry, College of Pharmacy, Kyoung Hee University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / pharmacology
Cell Differentiation / drug effects*
Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors*
Enzyme Activation / drug effects
Enzyme Inhibitors / pharmacology
Glutathione / metabolism
Humans
Isoquinolines / pharmacology
Naphthol AS D Esterase / analysis
Naphthoquinones / pharmacology*
Nitroblue Tetrazolium
Phagocytosis / drug effects
Protein Kinase C / antagonists & inhibitors*
Staurosporine / analogs & derivatives
Sulfonamides*
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Enzyme Inhibitors; 0/Isoquinolines; 0/Naphthoquinones; 0/Sulfonamides; 127243-85-0/H 89; 143086-33-3/N-ethoxycarbonyl-7-oxostaurosporine; 2797-51-5/2-chloro-3-amino-1,4-naphthoquinone; 298-83-9/Nitroblue Tetrazolium; 62996-74-1/Staurosporine; 70-18-8/Glutathione; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.13/Protein Kinase C; EC 3.1.-/Naphthol AS D Esterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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