Document Detail


Induction of diabetes in nonhuman primates by means of temporary arterial embolization and selective arterial injection of streptozotocin.
MedLine Citation:
PMID:  14695391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To develop and assess a technique for induction of C peptide-negative diabetes in adult nonhuman primates in preparation for preclinical investigation of type 1 diabetes treatments. MATERIALS AND METHODS: First, temporary embolization of the hepatic and gastric arteries was performed in 14 adult nonhuman primates (six cynomolgus, five rhesus, and three pigtail macaques). After embolization was confirmed with angiography, streptozotocin was injected at a dose of 50-70 mg/kg into the celiac artery and branches supplying the pancreas. The macaques then were given intravenous injections of arginine and glucose, and blood levels of insulin and C peptide were measured with an enzyme-linked immunosorbent assay to determine whether diabetes had been induced. RESULTS: All but one of the macaques developed persistent long-term C peptide-negative diabetes after the streptozotocin injection. One macaque did not develop diabetes after the initial injection and was given a second dose of streoptozotocin, which did induce diabetes. None of the macaques showed any symptoms of hepatic or renal injury, and only one died (of gastric dilatation 5 days after the procedure). CONCLUSION: Streptozotocin injection after temporary embolization of the hepatic and gastric arteries is a safe and reproducible method for inducing C peptide-negative diabetes in adult nonhuman primates in preparation for preclinical investigation of type 1 diabetes treatments.
Authors:
Michael G Tal; Boaz Hirshberg; Ziv Neeman; David Bunnell; S Soleimanpour; John Bacher; Noelle Patterson; Richard Chang; David M Harlan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Radiology     Volume:  230     ISSN:  0033-8419     ISO Abbreviation:  Radiology     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-25     Completed Date:  2004-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401260     Medline TA:  Radiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  163-8     Citation Subset:  AIM; IM    
Copyright Information:
Copyright RSNA, 2003
Affiliation:
Department of Diagnostic Imaging, Yale University School of Medicine, PO Box 208042, New Haven, CT 06520-8042, USA. michael.tal@yale.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Diabetes Mellitus, Experimental / etiology*
Embolization, Therapeutic
Injections, Intra-Arterial
Primates
Streptozocin / administration & dosage
Time Factors
Chemical
Reg. No./Substance:
18883-66-4/Streptozocin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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