| Induction of dendritic cell maturation by β-glucan isolated from Sparassis crispa. | |
| | |
MedLine Citation:
|
PMID: 20699131 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sparassis crispa is a medicinal mushroom containing high 6-branched 1,3-β-D-glucan (sparan) content, which exhibits immune-mediated antitumor activity. In the present study, we investigated the stimulating effect of sparan on phenotypic and functional maturation of dendritic cells (DCs). Phenotypic maturation was confirmed by the elevated expressions of CD40, CD80, CD86, and MHC-I/II molecules. Functional activation was proved by increased cytokine production of IL-12, IL-1β, TNF-α, and IFN-α/β, enhanced IL-2 production and proliferation of allogenic T cells, and decreased endocytosis. The role of toll-like receptor 4 (TLR4) as a membrane receptor of sparan was proved by the impaired maturation of DCs generated from bone marrow cells of tlr4⁻/⁻ knock-out mice and TLR4-mutated C3H/HeJ mice, and by using anti-MD-2/TLR4 neutralizing antibody. Sparan increased phosphorylation of ERK, p38, and JNK, and enhanced nuclear translocation of NF-κB p50/p65 in DCs. These results indicate that sparan activates DCs via MAPK and NF-κB signaling pathways, which are signaling molecules downstream of TLR4. |
| | |
Authors:
|
Hyung Sook Kim; Jee Youn Kim; Hwa Sun Ryu; Hyuk-Gu Park; Yong Ook Kim; Jong Soon Kang; Hwan Mook Kim; Jin Tae Hong; Youngsoo Kim; Sang-Bae Han |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-09 |
Journal Detail:
|
Title: International immunopharmacology Volume: 10 ISSN: 1878-1705 ISO Abbreviation: Int. Immunopharmacol. Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-10-04 Completed Date: 2011-02-15 Revised Date: 2011-04-29 |
Medline Journal Info:
|
Nlm Unique ID: 100965259 Medline TA: Int Immunopharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1284-94 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
College of Pharmacy and Medical Research Center (CICT), Chungbuk National University, 410 Seongbong, Heungduk, Cheongju, Chungbuk 361-763, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agaricales
/
chemistry* Animals Antibodies, Monoclonal Bone Marrow Cells Dendritic Cells / cytology*, drug effects*, physiology Female Glucans / pharmacology* Mice Mice, Inbred C57BL Mice, Knockout Specific Pathogen-Free Organisms Spleen / cytology Toll-Like Receptor 4 / genetics, metabolism beta-Glucans / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Glucans; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 4; 0/beta-Glucans; 0/sparan |
| Comments/Corrections | |
Comment In:
|
Int Immunopharmacol. 2011 Apr;11(4):529; author reply 530-1
[PMID:
21310257
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Platform technology to deliver prophylactic molecules orally: an example using the Class A select ag...
Next Document: Technological advances in mouse development studies. Preface.