Document Detail


Induction of the d-Amino Acid Oxidase from Trigonopsis variabilis.
MedLine Citation:
PMID:  16535339     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Induction of the d-amino acid oxidase (EC. 1.4.3.3) from the yeast Trigonopsis variabilis was investigated by using a minimal medium containing glucose as the carbon and energy source, (NH(inf4))(inf2)SO(inf4) as the nitrogen source, and various d- and dl-amino acid derivatives as inducers. The best new inducers found were N-carbamoyl-d-alanine, N-acetyl-d-tryptophan, and N-chloroacetyl-d-(alpha)-aminobutyric acid; when the induction effects of these compounds were compared with the effects of d-alanine as the nitrogen source and inducer, the resulting activities of d-amino acid oxidase per gram of dried yeast were 4.2, 2.1, and 1.5 times higher, respectively. The optimum concentration of the best inducer, N-carbamoyl-d-alanine, was 5 mM. This inducer could also be used in its racemic form. The induction was pH dependent. After cultivation of the yeast in a 50-liter bioreactor, d-amino acid oxidase activity of about 3,850 (mu)kat (231,000 U) was obtained. In addition, production of the d-amino acid oxidase was found to be significantly dependent on the metal salt composition of the medium. Addition of zinc ions was required to obtain high d-amino acid oxidase levels in the cells. The optimum concentration of ZnSO(inf4) was about 140 (mu)M.
Authors:
R Horner; F Wagner; L Fischer
Related Documents :
17841979 - Differential enzymatic activity in ecological races of typha latifolia l.
18985489 - Hydrocaffeic and p-coumaric acids, natural phenolic compounds, inhibit uv-b damage in w...
3964299 - Dissociation of increased lauric acid omega-hydroxylase activity from the antilipidemic...
2408419 - Production of 6-hydroxydopa by human tyrosinase.
11453999 - Structure and characterization of the glycan moiety of l-amino-acid oxidase from the ma...
21094199 - Modifications of the organic and mineral fractions of dental tissues following conditio...
156639 - Effects of phospholipase a2 and albumin on the calcium-dependent atpase and the lipid c...
1669669 - Taurine decreases fecal fatty acid and sterol excretion in cystic fibrosis. a randomize...
23666029 - 2-ketogluconic acid secretion by incorporation of pseudomonas putida kt 2440 gluconate ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-06-25     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2106-10     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A Role for Fructose 1,6-Diphosphate in the ATPase-Mediated Energy-Spilling Reaction of Streptococcus...
Next Document:  Automated Systems for Identification of Heterotrophic Marine Bacteria on the Basis of Their Fatty Ac...