Document Detail

Induction of cytochrome(s) P450-dependent drug metabolism in cultured MH1C1 hepatoma cells.
MedLine Citation:
PMID:  6518625     Owner:  NLM     Status:  MEDLINE    
A cell line derived from a Morris hepatoma, MH1C1, was examined for its in vitro expression of monooxygenases. These cells were found to contain different forms of cytochrome P450, as shown by the response to inducers, namely phenobarbital (PB), 3-methylcholanthrene (MC) and metyrapone (MP). MH1C1 cell monolayers exposed to PB or MC showed an increase in the concentration of two spectrally distinct forms of cytochrome P450. The PB and MC treatments elicited enzyme activities towards the substrates aminopyrine and benzo(a)pyrene, respectively. The cell treatment with metyrapone led to a simultaneous stimulation of aminopyrine demethylase and benzo(a)pyrene hydroxylase activities, so underlining the peculiar features of this inducer.
M Ferro; A M Bassi; U M Marinari; G Nanni; E Chiarpotto; G Poli; M U Dianzani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  2     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-03-14     Completed Date:  1985-03-14     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  263-8     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cytochrome P-450 Enzyme System / metabolism*
Cytochromes / metabolism
Enzyme Induction / drug effects
Liver Neoplasms, Experimental / enzymology*
Methylcholanthrene / pharmacology
Metyrapone / pharmacology
Mixed Function Oxygenases / metabolism*
Phenobarbital / pharmacology
Spectrum Analysis
Reg. No./Substance:
0/Cytochromes; 56-49-5/Methylcholanthrene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC protein, rat; YQE403BP4D/Phenobarbital; ZS9KD92H6V/Metyrapone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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