Document Detail


Induction of cytochrome(s) P450-dependent drug metabolism in cultured MH1C1 hepatoma cells.
MedLine Citation:
PMID:  6518625     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cell line derived from a Morris hepatoma, MH1C1, was examined for its in vitro expression of monooxygenases. These cells were found to contain different forms of cytochrome P450, as shown by the response to inducers, namely phenobarbital (PB), 3-methylcholanthrene (MC) and metyrapone (MP). MH1C1 cell monolayers exposed to PB or MC showed an increase in the concentration of two spectrally distinct forms of cytochrome P450. The PB and MC treatments elicited enzyme activities towards the substrates aminopyrine and benzo(a)pyrene, respectively. The cell treatment with metyrapone led to a simultaneous stimulation of aminopyrine demethylase and benzo(a)pyrene hydroxylase activities, so underlining the peculiar features of this inducer.
Authors:
M Ferro; A M Bassi; U M Marinari; G Nanni; E Chiarpotto; G Poli; M U Dianzani
Related Documents :
5064225 - Cell interactions in the immune response in vitro. 3. specific collaboration across a c...
127415 - Employment of synchronized cells and flow microfluorometry in investigations on the jb-...
10738105 - Influence of melatonin on proliferation and antioxidant system in ehrlich ascites carci...
3788475 - Incorporation of leucine by mouse synovial cells in vivo.
1577065 - Inhibitory and stimulatory signaling via immunoglobulin receptors: dichotomous response...
3016165 - A combination of four cell types for rapid detection of enteroviruses in clinical speci...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  2     ISSN:  0263-6484     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1985-03-14     Completed Date:  1985-03-14     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  263-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Cell Line
Cytochrome P-450 Enzyme System / metabolism*
Cytochromes / metabolism
Enzyme Induction / drug effects
Liver Neoplasms, Experimental / enzymology*
Methylcholanthrene / pharmacology
Metyrapone / pharmacology
Mixed Function Oxygenases / metabolism*
Phenobarbital / pharmacology
Rats
Spectrum Analysis
Chemical
Reg. No./Substance:
0/Cytochromes; 56-49-5/Methylcholanthrene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.14.14.1/Cyp1a2 protein, rat; YQE403BP4D/Phenobarbital; ZS9KD92H6V/Metyrapone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  DNA-damaging activity of biotic and xenobiotic aldehydes in Chinese hamster ovary cells.
Next Document:  Quantitative and qualitative changes of plasma membrane glycoproteins in the early period of liver r...