Document Detail


Induction of cytochrome P-450c in hematopoietic cells of fetal liver.
MedLine Citation:
PMID:  3541928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transplacental inductive effect of beta-naphthoflavone (beta NF) on cytochrome P-450 isozymes was studied in separate hematopoietic and hepatocyte cells from fetal rat liver. Two fractions of dispersed fetal liver cells were isolated by Ficoll-Paque centrifugation and shown by histologic examination to be enriched in erythroblasts and hepatocytes, respectively. beta NF treatment increased ethoxyresorufin-O-deethylase activity 250-fold in both erythroblast and hepatocyte cell fractions. Polyacrylamide gel electrophoresis and immunostaining techniques showed the induction of cytochrome P-450c, but not P-450d, in erythroblast and hepatocyte fractions.
Authors:
K T Shiverick; A G Kvello-Stenstrom; W H Donnelly; A S Salhab; J A Goldstein; M O James
Related Documents :
19296088 - Hypothermic storage of isolated human hepatocytes: a comparison between university of w...
8226108 - Experimentally induced colon cancer metastases in rat liver increase the proliferation ...
374218 - Immunofluorescence studies indicate that the basic trypsin-kallikrein-inhibitor of bovi...
20458738 - Differentiated human adipose-derived stem cells exhibit hepatogenic capability in vitro...
16806628 - Protocatechuic acid suppresses mpp+ -induced mitochondrial dysfunction and apoptotic ce...
9594378 - Ultrastructural and histochemical changes and apoptosis of inner enamel epithelium in r...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  141     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-02-04     Completed Date:  1987-02-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  299-305     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Benzoflavones / pharmacology
Cell Separation
Cytochrome P-450 CYP1A1
Cytochrome P-450 Enzyme System / biosynthesis*,  immunology
Enzyme Induction / drug effects
Hematopoietic Stem Cells / enzymology*
Immunosorbent Techniques
Liver / cytology,  embryology*,  enzymology
Microsomes, Liver / enzymology*
Oxidoreductases / metabolism
Rats
beta-Naphthoflavone
Grant Support
ID/Acronym/Agency:
ES-03766/ES/NIEHS NIH HHS; HD-18506/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Benzoflavones; 6051-87-2/beta-Naphthoflavone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Oxidoreductases; EC 1.14.14.1/Cytochrome P-450 CYP1A1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evidence for catalytic site cysteine and histidine by chemical modification of beta-hydroxy-beta-met...
Next Document:  Demonstration of immunohistochemical and immunochemical cross-reactivity of L-histidine and L-dopa d...