Document Detail

Induction of cyclooxygenase-1 in a human megakaryoblastic cell line (CMK) differentiated by phorbol ester.
MedLine Citation:
PMID:  9022759     Owner:  NLM     Status:  MEDLINE    
Human megakaryoblastic cells (CMK line) are known to differentiate to mature megakaryocyte-like cells by treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA). There are two isozymes of prostaglandin-forming cyclooxygenase enzyme. Constitutive cyclooxygenase-1 and inducible cyclooxygenase-2 were followed during differentiation of CMK cells. Treatment of the cells with 0.1 microM TPA for 4 days resulted in a 5-20-fold increase in cyclooxygenase activity. Northern and Western blot analyses revealed that cyclooxygenase-1 mRNA and protein increased in parallel with the enzyme activity. In contrast, cyclooxygenase-2 mRNA was detected only at 3 h. Furthermore, most of the increased cyclooxygenase activity was immunoprecipitated with anti-cyclooxygenase-1 antibody, and was not affected by a cyclooxygenase-2-specific inhibitor, NS-398. These results indicated that cyclooxygenase-1 rather than cyclooxygenase-2 was predominantly induced depending on TPA. The enzyme thus induced was localized by immunoelectron microscopy in nuclear envelope and endoplasmic reticulum of the CMK cells.
N Ueda; R Yamashita; S Yamamoto; K Ishimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1344     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-07     Completed Date:  1997-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  103-10     Citation Subset:  IM    
Department of Biochemistry, Tokushima University, School of Medicine, Japan.
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MeSH Terms
Arachidonic Acid / metabolism
Blotting, Northern
Blotting, Western
Cell Differentiation / drug effects*
Cyclooxygenase 1
Cyclooxygenase Inhibitors / pharmacology
Gene Expression Regulation, Developmental
Isoenzymes / metabolism*
Megakaryocytes / metabolism*
Membrane Proteins
Microscopy, Immunoelectron
Nitrobenzenes / pharmacology
Precipitin Tests
Prostaglandin-Endoperoxide Synthases / metabolism*
Prostaglandins / metabolism
RNA, Messenger / analysis,  metabolism
Sulfonamides / pharmacology
Tetradecanoylphorbol Acetate / pharmacology*
Reg. No./Substance:
0/Cyclooxygenase Inhibitors; 0/Isoenzymes; 0/Membrane Proteins; 0/Nitrobenzenes; 0/Prostaglandins; 0/RNA, Messenger; 0/Sulfonamides; 123653-11-2/N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; 16561-29-8/Tetradecanoylphorbol Acetate; 506-32-1/Arachidonic Acid; EC 1; EC protein, human; EC Synthases

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