Document Detail


Induction of chromosomal damage in CHO-K1 cells and their repair-deficient mutant XRS5 by X-ray and particle irradiation.
MedLine Citation:
PMID:  11542787     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytogenetic effects of X-rays and Au ions were investigated in repair-proficient CHO-K1 cells and their radiosensitive mutant strain xrs5, which shows a defect in the rejoining of DNA double-strand breaks. Both cell lines were synchronized by mitotic shake off, irradiated in G1-phase with either 250 kV X-rays or 780 MeV/u Au ions (LET: 1150 keV/micrometer) and chromosome aberrations were analyzed in first post-irradiation metaphases. Isoeffective doses of X-rays for the induction of aberrant cells and aberrations per cell were about 14 times lower for xrs5 than for CHO-K1 cells. After high LET radiation the difference in the cytogenetic response of both cell lines was drastically diminished. Furthermore, the analysis of the aberration types induced by sparsely and densely ionizing radiation showed for both cell lines specific changes in the spectrum of aberration types as LET increases. The experimental results are discussed with respect to the different types of lesions induced by sparsely and densely ionizing radiation.
Authors:
E Nasonova; S Ritter; T Fomenkova; G Kraft
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Advances in space research : the official journal of the Committee on Space Research (COSPAR)     Volume:  22     ISSN:  0273-1177     ISO Abbreviation:  Adv Space Res     Publication Date:  1998  
Date Detail:
Created Date:  2000-04-08     Completed Date:  2000-04-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9878935     Medline TA:  Adv Space Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  569-78     Citation Subset:  S    
Affiliation:
Joint Institute for Nuclear Research, Laboratory of Nuclear Problems, Dubna, Russia.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells / cytology,  radiation effects*
Cell Cycle / radiation effects*
Chromatids / radiation effects
Chromosome Aberrations*
Cosmic Radiation
Cricetinae
DNA Repair / genetics*
Gold
Heavy Ions*
Linear Energy Transfer
Mutation
Time Factors
X-Rays*
Grant Support
ID/Acronym/Agency:
F13P CT92 0064/CT/CIT NIH HHS
Chemical
Reg. No./Substance:
7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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