Document Detail


Induction and characterization of adventitious roots directly from the explants of Panax notoginseng.
MedLine Citation:
PMID:  16314969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adventitious roots from leafstalks and lateral roots were obtained directly from explants of Panax notoginseng. The lateral root explants were more sensitive to the induction of adventitious roots using indole-3-butyric acid. HPLC analysis of saponins extracted from the adventitious roots indicated that several protopanaxatriol saponins were present but ginsenoside Rd was missing, compared with the saponins extracted from the raw herbs. The dry weight of primary adventitious root culture of Panax notoginseng increased 5.25 times during multiplication in a classical shaking-flask system, suggesting that it is a culture system with great potential for scale-up.
Authors:
Xianfu Gao; Chunbao Zhu; Wei Jia; Wenyuan Gao; Mingfeng Qiu; Yongyu Zhang; Peigen Xiao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology letters     Volume:  27     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-29     Completed Date:  2006-02-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1771-5     Citation Subset:  IM    
Affiliation:
School of Pharmacy, Shanghai Jiao Tong University, 200030, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / methods*
Cell Proliferation / drug effects
Indoles / pharmacology
Panax / drug effects,  growth & development*,  metabolism*
Plant Roots / drug effects,  growth & development*,  metabolism*
Saponins / biosynthesis*
Chemical
Reg. No./Substance:
0/Indoles; 0/Saponins; 133-32-4/indolebutyric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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