Document Detail

Induction of cellular functions in spontaneously immortalized rat-2 cells transfected with cloned herpes simplex virus type 2 (HSV-2) DNA.
MedLine Citation:
PMID:  3026679     Owner:  NLM     Status:  MEDLINE    
Experiments were done to determine if cloned transforming sequences from herpes simplex virus type 2 (HSV-2) DNA confer upon transfected Rat-2 cells the capacity to be stimulated in phospholipase and cyclooxygenase activities following 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment. Tumor-derived Rat-2 cells transformed with sub-fragments (BamHI-E, HindIII/HpaI-ED, or PstI-C) overlapping the right-hand end of the BglII-C transforming region of HSV-2 DNA were stimulated by TPA in both phospholipase activity, measured by deacylation of arachidonic acid, and cyclooxygenase activity, measured by prostaglandin synthesis. Non-transfected Rat-2 control cells showed no increase in these enzyme activities following TPA treatment. To our knowledge, this is the first evidence that cloned HSV-2 DNA has the capacity to induce cellular functions (phospholipase and cyclooxygenase) in transformed mammalian cells.
C R Krebs; M Waite; R Jariwalla; L S Kucera
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  8     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-02-20     Completed Date:  1987-02-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-5     Citation Subset:  IM    
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MeSH Terms
Cell Transformation, Viral
Cloning, Molecular
DNA Restriction Enzymes / pharmacology
DNA, Viral / metabolism*
Phospholipases / metabolism
Prostaglandin-Endoperoxide Synthases / metabolism
Simplexvirus / genetics*
Tetradecanoylphorbol Acetate / pharmacology
Grant Support
Reg. No./Substance:
0/DNA, Viral; 16561-29-8/Tetradecanoylphorbol Acetate; EC Synthases; EC 3.1.-/Phospholipases; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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