Document Detail


Induction of cellular deoxyribonucleic acid synthesis in butyrate-treated cells by simian virus 40 deoxyribonucleic acid.
MedLine Citation:
PMID:  6180295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sodium butyrate (3 mM) inhibited the entry into the S phase of quiescent 3T3 cells stimulated by serum, but had no effect on the accumulation of cellular ribonucleic acid. Simian virus 40 infection or manual microinjection of cloned fragments from the simian virus 40 A gene caused quiescent 3T3 cells to enter the S phase even in the presence of butyrate. NGI cells, a line of 3T3 cells transformed by simian virus 40, grew vigorously in 3 mM butyrate. Homokaryons were formed between G1 and S-phase 3T3 cells, Butyrate inhibited the induction of deoxyribonucleic acid synthesis that usually occurs in B1 nuclei when G1 cells are fused with S-phase cells. However, when G1 3T3 cells were fused with exponentially growing NGI cells, the 3T3 nuclei were induced to enter deoxyribonucleic acid synthesis. In tsAF8 cells, a ribonucleic acid polymerase II mutant that stops in the G1 phase of the cell cycle, no temporal sequence was demonstrated between the butyrate block and the temperature-sensitive block. These results confirm previous reports that certain virally coded proteins can induce cell deoxyribonucleic acid synthesis in the absence of cellular functions that are required by serum-stimulated cells. Our interpretation of these data is that butyrate inhibited cell growth by inhibiting the expression of genes required for the G0 leads to G1 leads to S transition and that the product of the simian virus 40 A gene overrode this inhibition by providing all of the necessary functions for the entry into the S phase.
Authors:
S Kawasaki; L Diamond; R Baserga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  1     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-10-21     Completed Date:  1982-10-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1038-47     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Butyrates / pharmacology*
Cattle / blood
Cell Cycle / drug effects
Cell Transformation, Viral
Cells, Cultured
DNA / biosynthesis*
DNA, Recombinant
DNA, Viral / metabolism*
Hybrid Cells
Mice
Mice, Inbred Strains
Mutation
RNA / biosynthesis
Simian virus 40 / genetics*
Temperature
Chemical
Reg. No./Substance:
0/Butyrates; 0/DNA, Recombinant; 0/DNA, Viral; 63231-63-0/RNA; 9007-49-2/DNA
Comments/Corrections

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