| Induction of cell surface insulin antigenicity in fibroblasts transfected with islet genomic DNA. | |
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MedLine Citation:
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PMID: 3527753 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ltk- cells were transfected with total genomic DNA obtained from rat islets or from insulinoma cells mixed with DNA tagged with fluorescent dye. Fluorescence-activated cell sorter (FACS) was used for initial selection of successful transfectants, monitoring fluorescent DNA incorporated into the cell. For subsequent selections the cells were treated with anti-insulin antiserum labeled with fluorescent dye and selected by FACS. Beta-cell DNA, but not DNA from murine leukemic cells, induced the appearance of cell surface insulin antigenicity in fibroblasts. This phenotypic expression was transient. Together with our previous demonstration of the induction of insulin secretion in Ltk- cells [(1986) Biochem. Biophys. Res. Commun. 136, 638-644], these results indicate that beta-cell-specific characteristics can be transfected to non-endocrine cells by genomic DNA transfection. |
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Authors:
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B Y Klein; R Nesher; E Cerasi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 205 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1986-10-23 Completed Date: 1986-10-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 341-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens / analysis* Cell Separation DNA / genetics* DNA, Neoplasm / genetics Flow Cytometry Insulin / analysis*, immunology, secretion Insulinoma / genetics Islets of Langerhans / analysis L Cells (Cell Line) / analysis, immunology* Leukemia, Experimental Male Pancreatic Neoplasms / genetics Rats Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/DNA, Neoplasm; 11061-68-0/Insulin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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