Document Detail

Induction of cell surface insulin antigenicity in fibroblasts transfected with islet genomic DNA.
MedLine Citation:
PMID:  3527753     Owner:  NLM     Status:  MEDLINE    
Ltk- cells were transfected with total genomic DNA obtained from rat islets or from insulinoma cells mixed with DNA tagged with fluorescent dye. Fluorescence-activated cell sorter (FACS) was used for initial selection of successful transfectants, monitoring fluorescent DNA incorporated into the cell. For subsequent selections the cells were treated with anti-insulin antiserum labeled with fluorescent dye and selected by FACS. Beta-cell DNA, but not DNA from murine leukemic cells, induced the appearance of cell surface insulin antigenicity in fibroblasts. This phenotypic expression was transient. Together with our previous demonstration of the induction of insulin secretion in Ltk- cells [(1986) Biochem. Biophys. Res. Commun. 136, 638-644], these results indicate that beta-cell-specific characteristics can be transfected to non-endocrine cells by genomic DNA transfection.
B Y Klein; R Nesher; E Cerasi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  205     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-10-23     Completed Date:  1986-10-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
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MeSH Terms
Antigens / analysis*
Cell Separation
DNA / genetics*
DNA, Neoplasm / genetics
Flow Cytometry
Insulin / analysis*,  immunology,  secretion
Insulinoma / genetics
Islets of Langerhans / analysis
L Cells (Cell Line) / analysis,  immunology*
Leukemia, Experimental
Pancreatic Neoplasms / genetics
Reg. No./Substance:
0/Antigens; 0/DNA, Neoplasm; 11061-68-0/Insulin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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