Document Detail


Induction of cell signaling events by the cholera toxin B subunit in antigen-presenting cells.
MedLine Citation:
PMID:  17353279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cholera toxin (CT) is one of the most effective and widely studied mucosal adjuvants. Although the ADP-ribosylating A subunit has been implicated in augmenting immune responses, the receptor-binding B subunit (CT-B) has greater immunogenicity and may be a repository of adjuvant activity without potential toxicity. In order to elucidate mechanisms of immune modulation by CT-B alone, primary B cells and macrophages were assessed for responses to CT-B in vitro, as measured by the expression of cell surface markers, cellular signaling events, and cytokine secretion. Increased phosphorylation of multiple signaling molecules, including Erk1/2 and p38, was detected. CT-B also induced transactivation of the transcription elements cyclic AMP-responsive element and NF-kappaB, the latter of which was inhibited by phosphotyrosine inhibition. While specific inhibition of MEK1/2 did not reduce CT-B induction of cell surface marker expression, it did attenuate CT-B-mediated interleukin-6 secretion. These data show that CT-B induces a set of signaling events related to cellular activation, surface molecule expression, and cytokine production that has potential implications for elucidating CT-B adjuvant activity in the absence of enzymatically active holotoxin.
Authors:
Aletta C Schnitzler; Jennifer M Burke; Lee M Wetzler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-12
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-08-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3150-9     Citation Subset:  IM    
Affiliation:
Evans Biomedical Research Center, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Presenting Cells / drug effects*,  physiology
B-Lymphocytes / drug effects*,  immunology,  physiology
Cell Communication
Cholera Toxin / chemistry,  pharmacology*
Mice
Mice, Inbred C57BL
Signal Transduction / drug effects*,  physiology
Chemical
Reg. No./Substance:
9012-63-9/Cholera Toxin
Comments/Corrections

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