| Induction of cell cycle regulatory proteins by murine B cell proliferating pectic polysaccharide from the roots of Bupleurum falcatum L. | |
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MedLine Citation:
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PMID: 14556967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bupleuran 2IIc, a pectic polysaccharide isolated from the roots of Bupleurum falcatum L., was characterized as a T-cell-independent B cell mitogen, that activates, proliferates and differentiates B cells in vivo and in vitro (Immunology 97 (1999) 540). Studies were focused on elucidating the mechanism by which bupleuran 2IIc causes proliferation of B cells and expression of cell cycle regulatory proteins. B cells showed slower rates of entry into the S and G2/M phases of the cell cycle when stimulated with bupleuran 2IIc versus anti-IgM. However, the Stimulation Index continued up to two times longer with bupleuran 2IIc over anti-IgM. Although both bupleuran 2IIc and anti-IgM induced similar expressions of cell cycle regulatory proteins, cyclins D2, A, and B1, in B cells, those cells stimulated with bupleuran 2IIc appeared to sustain expressions of these protein for longer periods of time. Stimulation of B cells with bupleuran 2IIc induced phosphorylation of retinoblastoma protein, pRB, an important gene product regulating the restriction point, R, which is responsible for the transition from the G0/G1 to the S phases of the cell cycle. The results of this study demonstrate that both bupleuran 2IIc and anti-IgM interact with B cells, thus, leading to expressions of cell cycle regulatory proteins. However, the respective modes of binding and proximity of interactions with the B cell membrane may differ. |
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Authors:
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Tsukasa Matsumoto; Ying-Jie Guo; Takashi Ikejima; Haruki Yamada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunology letters Volume: 89 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-14 Completed Date: 2004-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 111-8 Citation Subset: IM |
Affiliation:
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Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, 108-8641, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / immunology, metabolism* Bupleurum / metabolism* Cell Cycle Proteins / biosynthesis, genetics* Cell Division / immunology, physiology Female Gene Expression Regulation Immunoglobulin M / immunology Mice Pectins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Immunoglobulin M; 0/Pectins; 138726-08-6/Bupleuran 2IIc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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