| Induction of carbon monoxide in donor animals prior to organ procurement reduces graft immunogenicity and inhibits chronic allograft dysfunction. | |
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MedLine Citation:
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PMID: 17038910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Nonspecific inflammatory damages occurring prior to organ transplantation reduce long-term graft survival. Here, we tested the beneficial effects of carbon monoxide (CO) induction by methylene chloride (MC). METHODS: Fischer-344 (F-344 Rat) or Dark Agouti (DA Rat) donor animals were either treated with MC four hours prior to organ removal or remained untreated. Kidneys were transplanted into Lewis (LEW) recipients. The low responder strain combination (F-344-->LEW) was studied for long-term graft changes. Dendritic cells (DCs) migration and early changes were followed in additional groups of a high responding donor/recipient strain combination (DA-->LEW). Native kidneys of uninephrectomized, age-matched normal animals served as controls. RESULTS: Following MC application COHb peaked within two hours in donor animals. Renal function and morphology improved significantly in renal allografts of CO induced donor animals and were comparable to native controls long-term (24 wks). Early after transplantation (24 hr) donor-derived DCs, CD4+ T-cells and alloreactive T-cells were significantly reduced following the engraftment of organs from treated donors. In addition, a trend towards a Th1/Th2 shift and a significant intragraft reduction of CD3 mRNA expression was observed. CONCLUSION: Donor treatment for the induction of CO reduced graft immunogenicity and inhibited chronic allograft nephropathy. |
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Authors:
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Paulo Ney Aguiar Martins; Anja Reutzel-Selke; Anke Jurisch; Christian Denecke; Kirstin Attrot; Andreas Pascher; Katja Kotsch; Johann Pratschke; Peter Neuhaus; Hans-Dieter Volk; Stefan G Tullius |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation Volume: 82 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-13 Completed Date: 2006-11-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: United States |
Other Details:
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Languages: eng Pagination: 938-44 Citation Subset: IM |
Affiliation:
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Division of Transplant Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD3 / genetics Carbon Monoxide / metabolism* Carboxyhemoglobin / metabolism Cytokines / genetics Dendritic Cells / drug effects, immunology Enzyme-Linked Immunosorbent Assay Flow Cytometry Inflammation Kidney Function Tests Kidney Transplantation / immunology, physiology* Methylene Chloride / pharmacology* RNA, Messenger / genetics Rats Rats, Inbred F344 Rats, Inbred Lew Reverse Transcriptase Polymerase Chain Reaction Tissue and Organ Harvesting / methods* Transplantation, Homologous / immunology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD3; 0/Cytokines; 0/RNA, Messenger; 630-08-0/Carbon Monoxide; 75-09-2/Methylene Chloride; 9061-29-4/Carboxyhemoglobin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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