Document Detail

Induction of the c-jun protooncogene expression by areca nut extract and arecoline on oral mucosal fibroblasts.
MedLine Citation:
PMID:  10964049     Owner:  NLM     Status:  MEDLINE    
To investigate the mechanisms of areca quid (AQ)-induced carcinogenesis, expression of c-fos and c-jun protooncogenes was examined in human oral mucosal fibroblasts after exposure to areca nut extracts (ANE) or arecoline. We found that treatment of cells with 200 microg/ml ANE or 10 microg/ml arecoline for 1 h induced about three-fold increase in c-jun mRNA levels. This increase was transient and the level of c-jun mRNAs returned rapidly to control levels thereafter. However, ANE and arecoline did not induce c-fos mRNA expression at detectable levels. During AQ chewing, oral mucosal cells are continuously stimulated by ANE and arecoline. Persistent induction of the c-jun protooncogene by ANE and arecoline may be one of the mechanisms in the carcinogenesis of oral squamous cell carcinoma in Taiwan. Furthermore, we observed that pre-incubation of cells with either N-acetyl-cysteine [a glutathione (GSH) precursor] or L-buthionine-S,R-sulfoximine (a specific inhibitor of GSH biosynthesis) had a minimal effect on arecoline-induced c-jun expression. Therefore, arecoline-induced c-jun expression is independent of GSH depletion.
T J Ho; C P Chiang; C Y Hong; S H Kok; Y S Kuo; M Yen-Ping Kuo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral oncology     Volume:  36     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-12-14     Completed Date:  2000-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  432-6     Citation Subset:  IM    
School of Dentistry, College of Medicine, National Taiwan University, 1 Chang-Te Street, 100, Taipei, Taiwan.
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MeSH Terms
Areca / adverse effects*
Arecoline / adverse effects*
Carcinoma, Squamous Cell / chemically induced*,  genetics
Fibroblasts / drug effects*
Gene Expression / drug effects
Mouth Mucosa / drug effects
Mouth Neoplasms / chemically induced*,  epidemiology,  genetics
Mutagenicity Tests
Plants, Medicinal*
Proto-Oncogene Proteins c-jun / drug effects*
Taiwan / epidemiology,  ethnology
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 63-75-2/Arecoline

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