| Induction of c-fos messenger ribonucleic acid in neuropeptide Y and growth hormone (GH)-releasing factor neurons in the rat arcuate nucleus following systemic injection of the GH secretagogue, GH-releasing peptide-6. | |
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MedLine Citation:
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PMID: 9003014 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we investigated the neurochemical identity of the arcuate cells activated following GH-releasing peptide-6 (GHRP-6) injection by comparing, on consecutive sections, the distribution c-fos messenger RNA (mRNA) with that of mRNAs for peptides synthesized in arcuate cells, including neuropeptide Y (NPY), GH-releasing factor (GRF), tyrosine hydroxylase, POMC, and somatostatin. Rats bearing chronically implanted jugular catheters were injected with either 50 micrograms GHRP-6 or vehicle. Thirty minutes later they were terminally anesthetized and perfused with fixative. Paraffin-embedded sections of 7 microns thickness were processed using in situ hybridization for either c-fos mRNA or mRNAs for the neurochemical markers. In GHRP-6-treated rats the mean (+/-SEM) number of cells expressing c-fos mRNA in the arcuate nucleus (23 +/- 2 cells/section per rat; n = 5) was significantly higher than for vehicle-treated controls (2 +/- 1 cells/section per rat; n = 5; P < 0.001, Mann-Whitney U test). Superimposed camera lucida maps indicated that, in GHRP-6-injected rats, neurochemically identifiable cells expressing c-fos mRNA also express NPY mRNA (51 +/- 4%), GRF mRNA (23 +/- 1%) tyrosine hydroxylase mRNA (11 +/- 3%), POMC mRNA (11 +/- 2%), or somatostatin mRNA (4 +/- 1%). Thus, the majority of cells expressing c-fos mRNA following GHRP-6 injection are NPY and GRF-containing cells. |
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Authors:
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S L Dickson; S M Luckman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 138 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1997 Feb |
Date Detail:
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Created Date: 1997-02-18 Completed Date: 1997-02-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 771-7 Citation Subset: AIM; IM |
Affiliation:
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Anatomy and Human Biology Group, King's College London, United Kingdom. sld20@cam.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arcuate Nucleus / metabolism* Gene Expression / drug effects Genes, fos / genetics* Growth Hormone-Releasing Hormone / metabolism* In Situ Hybridization Male Neurons / metabolism Neuropeptide Y / metabolism* Oligopeptides / pharmacology* Pro-Opiomelanocortin / genetics RNA, Messenger / biosynthesis* Rats Rats, Wistar Tyrosine 3-Monooxygenase / genetics |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptide Y; 0/Oligopeptides; 0/RNA, Messenger; 66796-54-1/Pro-Opiomelanocortin; 87616-84-0/growth hormone releasing hexapeptide; 9034-39-3/Growth Hormone-Releasing Hormone; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
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