Document Detail

Induction of c-fos messenger ribonucleic acid in neuropeptide Y and growth hormone (GH)-releasing factor neurons in the rat arcuate nucleus following systemic injection of the GH secretagogue, GH-releasing peptide-6.
MedLine Citation:
PMID:  9003014     Owner:  NLM     Status:  MEDLINE    
In this study we investigated the neurochemical identity of the arcuate cells activated following GH-releasing peptide-6 (GHRP-6) injection by comparing, on consecutive sections, the distribution c-fos messenger RNA (mRNA) with that of mRNAs for peptides synthesized in arcuate cells, including neuropeptide Y (NPY), GH-releasing factor (GRF), tyrosine hydroxylase, POMC, and somatostatin. Rats bearing chronically implanted jugular catheters were injected with either 50 micrograms GHRP-6 or vehicle. Thirty minutes later they were terminally anesthetized and perfused with fixative. Paraffin-embedded sections of 7 microns thickness were processed using in situ hybridization for either c-fos mRNA or mRNAs for the neurochemical markers. In GHRP-6-treated rats the mean (+/-SEM) number of cells expressing c-fos mRNA in the arcuate nucleus (23 +/- 2 cells/section per rat; n = 5) was significantly higher than for vehicle-treated controls (2 +/- 1 cells/section per rat; n = 5; P < 0.001, Mann-Whitney U test). Superimposed camera lucida maps indicated that, in GHRP-6-injected rats, neurochemically identifiable cells expressing c-fos mRNA also express NPY mRNA (51 +/- 4%), GRF mRNA (23 +/- 1%) tyrosine hydroxylase mRNA (11 +/- 3%), POMC mRNA (11 +/- 2%), or somatostatin mRNA (4 +/- 1%). Thus, the majority of cells expressing c-fos mRNA following GHRP-6 injection are NPY and GRF-containing cells.
S L Dickson; S M Luckman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  138     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-02-18     Completed Date:  1997-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  771-7     Citation Subset:  AIM; IM    
Anatomy and Human Biology Group, King's College London, United Kingdom.
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MeSH Terms
Arcuate Nucleus / metabolism*
Gene Expression / drug effects
Genes, fos / genetics*
Growth Hormone-Releasing Hormone / metabolism*
In Situ Hybridization
Neurons / metabolism
Neuropeptide Y / metabolism*
Oligopeptides / pharmacology*
Pro-Opiomelanocortin / genetics
RNA, Messenger / biosynthesis*
Rats, Wistar
Tyrosine 3-Monooxygenase / genetics
Reg. No./Substance:
0/Neuropeptide Y; 0/Oligopeptides; 0/RNA, Messenger; 66796-54-1/Pro-Opiomelanocortin; 87616-84-0/growth hormone releasing hexapeptide; 9034-39-3/Growth Hormone-Releasing Hormone; EC 3-Monooxygenase

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