Document Detail

Induction of c-fos and c-jun protooncogenes expression by formaldehyde-releasing and epoxy resin-based root-canal sealers in human osteoblastic cells.
MedLine Citation:
PMID:  11774303     Owner:  NLM     Status:  MEDLINE    
An important requirement for a root-canal sealer is biologic compatibility; most evaluations have focused on general toxicological and local tissue irritating properties. There is only scant information about mutagenicity or carcinogenicity testing for root-canal sealer. It has been shown that c-fos and c-jun are induced rapidly by a variety of chemical and physical stimuli. Numerous works have extensively investigated the induction mechanisms of c-fos and c-jun protooncogenes by these agents; however, little is known about the induction of cellular signaling events and specific gene expression after cell exposure to root-canal sealers. Therefore, we used osteoblastic cell line U2-OS to examine the effect of zinc-oxide eugenol-based (N2 and Endomethasome), epoxy resin-based (AH Plus), and calcium hydroxide-based (Sealapex) root-canal sealers on the expression of c-fos and c-jun protooncogenes to understand in more detail the molecular mechanisms of root-canal sealer-induced genotoxicity. The cytotoxicity decreased in an order of N2 > Endomethasome > AH Plus > Sealapex. In addition, N2, Endomethasome, and AH Plus rapidly induced c-jun and c-fos mRNA levels in cells. However, Sealapex did not induce c-jun and c-fos mRNA expression at detectable levels all time points. Taken together, persistent induction of c-jun and c-fos protooncogenes by formaldehyde-releasing and epoxy resin-based root-canal sealers may be distributed systemically via apex to cause some unexpected adverse effects on human beings. These data should be taken into consideration when choosing a root-canal sealer.
Fu-Mei Huang; Yih-Shou Hsieh; Kuo-Wei Tai; Ming-Yung Chou; Yu-Chao Chang
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  59     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-01-04     Completed Date:  2003-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  460-5     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Inc.
Department of Dentistry, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan.
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MeSH Terms
Biocompatible Materials / pharmacology
Cell Survival / drug effects
Epoxy Resins
Gene Expression Regulation / drug effects*
Genes, fos / drug effects*,  genetics
Genes, jun / drug effects*,  genetics
Osteoblasts / drug effects*
RNA, Messenger / drug effects
Root Canal Filling Materials / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Biocompatible Materials; 0/Epoxy Resins; 0/RNA, Messenger; 0/Root Canal Filling Materials; 50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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