Document Detail


Induction of c-fos and c-jun gene expression by phenolic antioxidants.
MedLine Citation:
PMID:  8145765     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have found that phenolic antioxidants specifically induce expression of the c-fos and c-jun protooncogenes. After treatment of quiescent human hepatoma HepG2 cells with butylated hydroxytoluene, butylated hydroxyanisole, or other phenolic antoxidants, the levels of c-fos and c-jun mRNAs are substantially increased. This response is antioxidant specific, dose dependent, and transient, with maximal levels at 3-6 h. The antioxidant-specific induction of c-fos/CAT promoter constructs in transient transfections indicates that at least a portion of this response is transcriptional. Deletions and point mutations map sequences required for the antioxidant response of the c-fos promoter to the serum response element. The antioxidant-specific induction of expression directed by a reporter plasmid containing four AP-1 sites and the induction of AP-1 DNA-binding activity confirm previous results indicating that antioxidant treatment increases AP-1 activity.
Authors:
H S Choi; D D Moore
Related Documents :
12093805 - Role of ap-1 in the coordinate induction of rat glutamate-cysteine ligase and glutathio...
1900355 - Inhibition of pdgf-induced c-jun and c-fos expression by a tyrosine protein kinase inhi...
24074615 - Expression of microrna-29b2-c cluster is positively regulated by myod in l6 cells.
16380965 - Direct manipulation of activator protein-1 controls thymocyte proliferation in vitro.
20932785 - Mrna expression of canine atp10c, a p4-type atpase, is positively associated with body ...
2007095 - Expression of the jun-b gene during induction of monocytic differentiation.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  7     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-05-03     Completed Date:  1994-05-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1596-602     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Base Sequence
Butylated Hydroxyanisole / pharmacology
Butylated Hydroxytoluene / pharmacology
Gene Expression Regulation / drug effects*
Genes, fos / drug effects*
Genes, jun / drug effects*
Humans
Hydrogen Peroxide / pharmacology
Molecular Sequence Data
Oxidation-Reduction
Phenols / pharmacology*
Promoter Regions, Genetic / drug effects
Proto-Oncogene Proteins c-jun / metabolism
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Phenols; 0/Proto-Oncogene Proteins c-jun; 128-37-0/Butylated Hydroxytoluene; 25013-16-5/Butylated Hydroxyanisole; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Thyroid transcription factor 1 activates the promoter of the thyrotropin receptor gene.
Next Document:  Cloning of the rat progesterone receptor gene 5'-region and identification of two functionally disti...