Document Detail

Induction of c-fos and c-jun gene expression by phenolic antioxidants.
MedLine Citation:
PMID:  8145765     Owner:  NLM     Status:  MEDLINE    
We have found that phenolic antioxidants specifically induce expression of the c-fos and c-jun protooncogenes. After treatment of quiescent human hepatoma HepG2 cells with butylated hydroxytoluene, butylated hydroxyanisole, or other phenolic antoxidants, the levels of c-fos and c-jun mRNAs are substantially increased. This response is antioxidant specific, dose dependent, and transient, with maximal levels at 3-6 h. The antioxidant-specific induction of c-fos/CAT promoter constructs in transient transfections indicates that at least a portion of this response is transcriptional. Deletions and point mutations map sequences required for the antioxidant response of the c-fos promoter to the serum response element. The antioxidant-specific induction of expression directed by a reporter plasmid containing four AP-1 sites and the induction of AP-1 DNA-binding activity confirm previous results indicating that antioxidant treatment increases AP-1 activity.
H S Choi; D D Moore
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular endocrinology (Baltimore, Md.)     Volume:  7     ISSN:  0888-8809     ISO Abbreviation:  Mol. Endocrinol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-05-03     Completed Date:  1994-05-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8801431     Medline TA:  Mol Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1596-602     Citation Subset:  IM    
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
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MeSH Terms
Antioxidants / pharmacology*
Base Sequence
Butylated Hydroxyanisole / pharmacology
Butylated Hydroxytoluene / pharmacology
Gene Expression Regulation / drug effects*
Genes, fos / drug effects*
Genes, jun / drug effects*
Hydrogen Peroxide / pharmacology
Molecular Sequence Data
Phenols / pharmacology*
Promoter Regions, Genetic / drug effects
Proto-Oncogene Proteins c-jun / metabolism
Structure-Activity Relationship
Reg. No./Substance:
0/Antioxidants; 0/Phenols; 0/Proto-Oncogene Proteins c-jun; 128-37-0/Butylated Hydroxytoluene; 25013-16-5/Butylated Hydroxyanisole; 7722-84-1/Hydrogen Peroxide

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