| Induction of c-fos, calcitonin gene expression, and acidic fibroblast growth factor production in a multipeptide-secreting neuroendocrine cell line. | |
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MedLine Citation:
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PMID: 2893727 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The multipeptide-secreting 44-2C cell line maintains differentiated function when grown in a serum-free, growth factor- and hormone-deprived milieu. The cells continue to synthesize and secrete calcitonin (CT), CT gene-related peptide, neurotensin, and somatostatin and respond to cellular secretagogues such as GRF and acidic and basic fibroblast growth factor. We designed experiments to ascertain the functional role(s) of cellular factors involved in the maintenance of the differentiated state in 44-2C cells. We report here the phenotypic transformation that occurs in these cells in the course of adjustment to the serum-free state. We also show the differential increase in CT-specific mRNA, the transient induction of c-fos, and the characterization of biologically active acidic fibroblast growth factor. |
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Authors:
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F N Zeytin; S F Rusk; A Baird; V Raymond; S E Leff; T Haaparanta; A J Mandell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 122 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-04-06 Completed Date: 1988-04-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1114-20 Citation Subset: AIM; IM |
Affiliation:
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Laboratory for Neuroendocrinology, Salk Institute, La Jolla, California 92037. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Calcitonin / genetics*, secretion Calcitonin Gene-Related Peptide Cell Differentiation Cell Line Fibroblast Growth Factors / genetics*, pharmacology Gene Expression Regulation* / drug effects Growth Hormone-Releasing Hormone / pharmacology Neuropeptides / genetics Neurosecretory Systems / cytology, drug effects, physiology* Neurotensin / secretion Norepinephrine / pharmacology Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins c-fos RNA, Messenger / biosynthesis Rats Somatostatin / secretion |
| Grant Support | |
ID/Acronym/Agency:
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DK-18811/DK/NIDDK NIH HHS; HD-09690/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptides; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 39379-15-2/Neurotensin; 51-41-2/Norepinephrine; 51110-01-1/Somatostatin; 62031-54-3/Fibroblast Growth Factors; 83652-28-2/Calcitonin Gene-Related Peptide; 9007-12-9/Calcitonin; 9034-39-3/Growth Hormone-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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