Document Detail


Induction of c-Jun phosphorylation in spinal motoneurons in neonatal and adult rats following axonal injury.
MedLine Citation:
PMID:  20096669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study aims to address if phosphorylation of the transcription factor c-Jun is associated with lesion-induced death of spinal motoneurons, and if this cellular response is modulated by glial-cell-line-derived neurotrophic factor (GDNF). We found that after both distal axotomy and root avulsion, spinal motoneurons in neonatal rats expressed phosphorylated c-Jun (p-c-Jun) and almost all injured motoneurons in these animals died. Similarly, root avulsion in adult rats also induced p-c-Jun expression that preceded the loss of motoneurons. In contrast, neither motoneuron death nor p-c-Jun induction was found after distal axotomy of spinal nerves in adult rats. Application of GDNF after distal axotomy in the neonatal model prevented motoneuron death but did not alter the expression of p-c-Jun in the surviving motoneurons. We conclude that c-Jun phosphorylation correlates with the cellular events leading to motoneuron death and that its expression cannot be modulated by GDNF. We further showed that expression of p-c-Jun was not correlated with the expression of growth-associated protein-43 (GAP-43), whose expression was closely correlated both temporally and spatially with periods of axonal outgrowth, suggesting that p-c-Jun may not be related with axonal regeneration of injured motoneurons.
Authors:
Qiuju Yuan; Bing Hu; Yin Wu; Tak-Ho Chu; Huanxin Su; Weiming Zhang; Kwok-Fai So; Zhixiu Lin; Wutian Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-21
Journal Detail:
Title:  Brain research     Volume:  1320     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-22     Completed Date:  2010-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7-15     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism
Animals
Animals, Newborn
Axons / metabolism*
Cell Count
Cell Death / physiology
Cell Survival / physiology
Disease Models, Animal
Female
GAP-43 Protein / metabolism
Glial Cell Line-Derived Neurotrophic Factor / metabolism
Immunohistochemistry
Motor Neurons / metabolism*
Nerve Regeneration / physiology
Phosphorylation
Proto-Oncogene Proteins c-jun / metabolism*
Rats
Rats, Sprague-Dawley
Spinal Cord / metabolism*
Spinal Nerves / injuries*,  metabolism*
Chemical
Reg. No./Substance:
0/GAP-43 Protein; 0/Glial Cell Line-Derived Neurotrophic Factor; 0/Proto-Oncogene Proteins c-jun

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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