| Induction of blood lymphocyte cytochrome P450 2E1 in early stage alcoholic liver cirrhosis. | |
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MedLine Citation:
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PMID: 20843640 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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To validate the induction of blood lymphocyte cytochrome P450 2E1 (CYP2E1) expression in alcoholic liver cirrhosis and mRNA and protein expression of CYP2E1 in freshly prepared blood lymphocytes of alcoholic liver cirrhotic (ACP), nonalcoholic cirrhotic patients (NACP), alcoholic controls (ACs), and nonalcoholic controls (NACs) were investigated. Registered ACP and NACP patients at Sanjay Gandhi Postgraduate Institute of Medical Science, Lucknow, India along with NACs and ACs were included in the study. Real time polymerase chain reaction, enzyme-linked immunosorbent assay, and CYP2E1-dependent enzyme activity were determined in blood lymphocytes isolated from cases and controls. Significant increases in CYP2E1 mRNA and protein expression were observed in freshly prepared blood lymphocytes isolated from ACs and ACP patients as compared with respective NACs or NACP patients. A concomitant increase in N-nitrosodimethyamine demethylase activity was evident in the blood lymphocytes of ACs and ACP patients. Interestingly, the comparative increase observed in CYP2E1 expression was of greater magnitude in the blood lymphocytes isolated from ACP patients, although they abstained from alcohol drinking. Findings suggest that significant increase in the CYP2E1 mRNA and protein expression in the blood lymphocytes, isolated from early stage ACP patients, can be used to predict alcohol-induced toxicity. |
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Authors:
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Anwar Jamal Khan; Amit Sharma; Gourdas Choudhuri; Devendra Parmar |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-16 |
Journal Detail:
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Title: Alcohol (Fayetteville, N.Y.) Volume: 45 ISSN: 1873-6823 ISO Abbreviation: Alcohol Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-12-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502311 Medline TA: Alcohol Country: United States |
Other Details:
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Languages: eng Pagination: 81-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Developmental Toxicology Division, Indian Institute of Toxicology Research/CSIR, Lucknow, Uttar Pradesh, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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