Document Detail


Induction of axonal differentiation by silencing plasma membrane-associated sialidase Neu3 in neuroblastoma cells.
MedLine Citation:
PMID:  17176265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reduction of 70% of the plasma membrane-associated sialidase Neu3 activity, due to a corresponding reduction of the enzyme expression by transducing cells with a short hairpin RNA encoding a sequence target (complementary messenger of mouse Neu3), caused neurite elongation in Neuro2a murine neuroblastoma cells. The differentiation process was accompanied in parallel by an increase of the acetylcholinesterase activity, a moderate increase of the c-Src expression and by the presence of the axonal marker tau protein on the neurites. The sphingolipid pattern and turnover in transduced and control cells were characterized by thin layer chromatography, mass spectrometry and metabolic radiolabeling after feeding cells with tritiated sphingosine. Control cells contained about 2 nmol of gangliosides/mg cell protein. GM2 was the main compound, followed by GD1a, GM3 and GM1. In Neu3 silenced cells, the total ganglioside content remained quite similar, but GM2 increased by 54%, GM3 remain constant, and GM1 and GD1a decreased by 66% and 50%, respectively. Within the organic phase sphingolipids, ceramide decreased by 50%, whereas the sphingomyelin content did not change in Neu3 silenced cells.
Authors:
Rea Valaperta; Manuela Valsecchi; Federica Rocchetta; Massimo Aureli; Simona Prioni; Alessandro Prinetti; Vanna Chigorno; Sandro Sonnino
Related Documents :
9437015 - Mitochondrial membrane potential and nuclear changes in apoptosis caused by serum and n...
1315285 - Effects of ngf and glucocorticoid on ngf receptor immunolabeling of cultured rhesus adr...
14607315 - Baculovirus p35 gene greatly enhances pc12 cell's resistance against oxidative stress.
16233645 - Immobilization of arg-gly-asp (rgd) sequence in a thermosensitive hydrogel for cell del...
19513905 - Mitochondrial reactive oxygen species originating from romo1 exert an important role in...
17525485 - Cytokeratin ck20 is a sensitive marker for crooke's cells and the early cytoskeletal ch...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-14
Journal Detail:
Title:  Journal of neurochemistry     Volume:  100     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-03-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  708-19     Citation Subset:  IM    
Affiliation:
Department of Medical Chemistry, Biochemistry and Biotechnology, and Center of Excellence on Neurodegenerative Diseases, University of Milan, Segrate, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / metabolism
Animals
Cell Differentiation / genetics*
Cell Line, Tumor
Ceramides / metabolism
Down-Regulation / genetics
Gangliosides / metabolism
Gene Silencing / physiology
Growth Cones / metabolism*,  ultrastructure
Membrane Proteins / genetics*
Mice
Nervous System / cytology,  growth & development*,  metabolism*
Neuraminidase / genetics*
Neuroblastoma
RNA / genetics
Sphingolipids / metabolism
Transduction, Genetic
src-Family Kinases / metabolism
tau Proteins / metabolism
Chemical
Reg. No./Substance:
0/Ceramides; 0/Gangliosides; 0/Membrane Proteins; 0/Sphingolipids; 0/tau Proteins; 63231-63-0/RNA; EC 2.7.10.2/src-Family Kinases; EC 3.1.1.7/Acetylcholinesterase; EC 3.2.1.18/Neu3 protein, mouse; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Post-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier ...
Next Document:  Novel methodology for assessment of prophylactic platelet transfusion therapy by measuring increased...