| Induction of apoptotic cell death by mycelium extracts of Phellinus linteus in human neuroblastoma cells. | |
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MedLine Citation:
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PMID: 15254770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phellinus linteus is a well-known Oriental medicinal fungus that has various biological activities, including immunomodulatory and anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the effects of mycelium extracts of P. linteus (MEPL) on the growth of human neuroblastoma SK-N-MC cells. Upon treatment with MEPL, a concentration-dependent inhibition of cell proliferation was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and an increase in the sub-G1 population. The anti-proliferative and apoptotic effects of MEPL were associated with a marked induction of the Bax and cyclin-dependent kinase inhibitor p21. Western blotting and in vitro caspase-3 activity assay demonstrated that the processing/activation of caspases accompanies the generation of MEPL-mediating apoptotic cell death. In addition, the proteolytic cleavage of specific target proteins such as poly(ADP-ribose) polymerase and beta-catenin were observed. Taken together, the present results suggest that apoptotic signals evoked by MEPL in human neuroblastoma SK-N-MC cells may converge caspase-3 activation through an up-regulation of Bax rather than a down-regulation of Bcl-2. |
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Authors:
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Yung Hyun Choi; Man Kyu Huh; Chung-Ho Ryu; Byung Tae Choi; Young-Kee Jeong |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 14 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-07-15 Completed Date: 2005-03-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 227-32 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Korea. choiyh@deu.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Agaricales
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metabolism* Antineoplastic Agents / pharmacology Apoptosis* Blotting, Western Caspase 3 Caspases / metabolism Cell Cycle Proteins / metabolism Cell Line, Tumor Cell Nucleus / metabolism Cell Proliferation Chromatin / metabolism Coloring Agents / pharmacology Cyclin-Dependent Kinase Inhibitor p21 Dose-Response Relationship, Drug Down-Regulation Flow Cytometry G1 Phase Humans Immunoblotting Mycelium / metabolism* Neuroblastoma / drug therapy*, pathology* Proto-Oncogene Proteins c-bcl-2 / metabolism RNA / metabolism Reverse Transcriptase Polymerase Chain Reaction Tetrazolium Salts / pharmacology Thiazoles / pharmacology Time Factors bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/BAX protein, human; 0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Chromatin; 0/Coloring Agents; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tetrazolium Salts; 0/Thiazoles; 0/bcl-2-Associated X Protein; 298-93-1/thiazolyl blue; 63231-63-0/RNA; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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