| Induction of apoptosis and lipogenesis in human preadipocyte cell line by n-3 PUFAs. | |
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MedLine Citation:
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PMID: 20812919 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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We examined the effect of n-3 PUFAs (polyunsaturated fatty acids) on the growth and maturation of human preadipocyte cell line AML-I. On day 3 of the culture, n-3 fatty acids such as DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid), but not n-6 fatty acid LA (linoleic acid), induced growth arrest accompanied by the appearance of characteristics of apoptosis in AML-I cells at concentrations between 250 and 500 μM by Annexin V-FITC staining. In Western blotting analysis, the loss of NF-κB, Bcl-2 and p-Akt and the accumulation of Bad and Akt were observed in the cytoplasmic protein from the EPA-treated cells. Exposure of AML-I to EPA or DHA increased the cytoplasmic lipid accumulation compared with the vehicle-treated cells in a time-dependent manner during 4 and 6 days culture period by Oil Red O staining. The expression of FAS (fatty acid synthase) and PPAR-γ (peroxisome proliferator-activated receptor-γ) were increased in EPA-treated cells. These results suggest that EPA and DHA promote differentiation, inhibit proliferation and induce apoptosis in preadipocyte cell line AML-I. |
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Authors:
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Haruka Hanada; Keiko Morikawa; Kaori Hirota; Mitsuko Nonaka; Yukari Umehara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-12-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 51-9 Citation Subset: IM |
Affiliation:
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Division of Clinical Nutrition, Department of Nutritional Science, Sagami Womens University, 211 Bunkyo, Sagamihara, Kanagawa 2288533, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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