Document Detail

Induction of apoptosis and lipogenesis in human preadipocyte cell line by n-3 PUFAs.
MedLine Citation:
PMID:  20812919     Owner:  NLM     Status:  In-Data-Review    
We examined the effect of n-3 PUFAs (polyunsaturated fatty acids) on the growth and maturation of human preadipocyte cell line AML-I. On day 3 of the culture, n-3 fatty acids such as DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid), but not n-6 fatty acid LA (linoleic acid), induced growth arrest accompanied by the appearance of characteristics of apoptosis in AML-I cells at concentrations between 250 and 500 μM by Annexin V-FITC staining. In Western blotting analysis, the loss of NF-κB, Bcl-2 and p-Akt and the accumulation of Bad and Akt were observed in the cytoplasmic protein from the EPA-treated cells. Exposure of AML-I to EPA or DHA increased the cytoplasmic lipid accumulation compared with the vehicle-treated cells in a time-dependent manner during 4 and 6 days culture period by Oil Red O staining. The expression of FAS (fatty acid synthase) and PPAR-γ (peroxisome proliferator-activated receptor-γ) were increased in EPA-treated cells. These results suggest that EPA and DHA promote differentiation, inhibit proliferation and induce apoptosis in preadipocyte cell line AML-I.
Haruka Hanada; Keiko Morikawa; Kaori Hirota; Mitsuko Nonaka; Yukari Umehara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-12-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  51-9     Citation Subset:  IM    
Division of Clinical Nutrition, Department of Nutritional Science, Sagami Womens University, 211 Bunkyo, Sagamihara, Kanagawa 2288533, Japan.
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