Document Detail

Induction of apoptosis in human leukemia U937 cells by anthocyanins through down-regulation of Bcl-2 and activation of caspases.
MedLine Citation:
PMID:  19287965     Owner:  NLM     Status:  MEDLINE    
Anthocyanins are a class of flavonoids, widely spread throughout the plant kingdom, that exhibit important anti-oxidant and anti-inflammatory actions as well as chemotherapeutic effects. However, little is known concerning the molecular mechanisms by which these activities are exerted. In this study, we investigated the anthocyanins isolated from Vitis coignetiae Pulliat for their potential anti-proliferative and apoptotic effects on human leukemia U937 cells. It was found that these anthocyanins inhibit cell viability and induce apoptotic cell death of U937 cells in a dose-dependent manner, as measured by hemocytometer counts, by alteration in the mitochondrial membrane potential, by increases in sub-G1 populations and by DNA ladder formation. Apoptosis of U937 cells by anthocyanins was associated with modulation of expression of Bcl-2 and IAP family members. Consequently, anthocyanin treatment induced proteolytic activation of caspase-3, -8 and -9, and a concomitant degradation of poly(ADP-ribose) polymerase. However, anthocyanin-induced growth inhibition and apoptosis were significantly attenuated in Bcl-2 overexpressing U937 cells. Furthermore, z-DEVD-fmk, a caspase-3 specific inhibitor, blocked apoptosis and increased the survival of anthocyanin-treated U937 cells. Taken together, these results show that Bcl-2 and caspases are key regulators of apoptosis in response to anthocyanins in human leukemia U937 cells.
Shin Hwa Lee; Su Min Park; Suk Min Park; Jae Hyeon Park; Dong Yeok Shin; Gi Young Kim; Chung Ho Ryu; Sung Chul Shin; Jin Myung Jung; Ho Sung Kang; Won Sup Lee; Yung Hyun Choi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  34     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1077-83     Citation Subset:  IM    
Korea Science Academy, Busan 614-822, Korea.
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MeSH Terms
Anthocyanins / pharmacology*
Caspases / metabolism*
Cell Survival
DNA / metabolism
Enzyme Activation
Gene Expression Regulation, Enzymologic*
Gene Expression Regulation, Neoplastic*
Membrane Potentials
Mitochondrial Membranes / metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism*
U937 Cells
Vitis / metabolism
Reg. No./Substance:
0/Anthocyanins; 0/Proto-Oncogene Proteins c-bcl-2; 9007-49-2/DNA; EC 3.4.22.-/Caspases

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