| Induction of apoptosis in human leukemia K562 cells by cardiotoxin III. | |
| | |
MedLine Citation:
|
PMID: 15763081 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cardiotoxin III (CTX III), a basic polypeptide with 60 amino acid residues isolated from Naja naja atra venom, has been reported to have anticancer activity. CTX III was found to inhibit the growth of K562 cells in a time-and dose-dependent manner with IC50 value of 1.7 microg/ml, and it displayed several features of apoptosis including apoptotic body formation, increase of sub G1 population, DNA fragmentation and poly (ADP-ribose) polymerase (PARP) cleavage. Investigation of the mechanism of CTXIII--induced apoptosis revealed that the treatment of K562 cells with CTX III resulted in the activation of caspase-9, caspase-3 and subsequent cleavage of its substrate PARP and that CTXIII was also associated with an early release of cytochrome c from the mitochondria. These results suggest that CTX III may induce apoptosis through a mitochondria- and caspase-dependent mechanism. |
| | |
Authors:
|
Sheng-Huei Yang; Mei-Chin Lu; Ching-Ming Chien; Chia-Houg Tsai; Yu-Jhang Lu; Tzyh-Chyuan Hour; Shinne-Ren Lin |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-01-27 |
Journal Detail:
|
Title: Life sciences Volume: 76 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2005 Apr |
Date Detail:
|
Created Date: 2005-03-14 Completed Date: 2005-04-18 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
|
Languages: eng Pagination: 2513-22 Citation Subset: IM |
Affiliation:
|
Faculty of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan 807, ROC. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Blotting, Western Caspase 3 Caspase 9 Caspases / metabolism Cobra Cardiotoxin Proteins / toxicity* Cytochromes c / metabolism DNA Fragmentation / drug effects Dose-Response Relationship, Drug Enzyme Activation / drug effects Flow Cytometry Humans Inhibitory Concentration 50 K562 Cells Mitochondria / drug effects Poly(ADP-ribose) Polymerases / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Cobra Cardiotoxin Proteins; 0/cardiotoxin III, Naja naja atra; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Homocysteine alterations in experimental cholestasis and its subsequent cirrhosis.
Next Document: Monitoring microbial community in a subsurface soil contaminated with hydrocarbons by quinone profil...