| Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi Radix. | |
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MedLine Citation:
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PMID: 18955103 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the apoptotic effects of crude saponins acquired from the roots of Platycodon grandiflorum (SPR) in HT-29 human colon cancer cells. SPR decreased HT-29 cell proliferation in dose- and time-dependent manners by inducing apoptosis via DNA fragmentation and poly (ADP-ribose) polymerase (PARP) cleavage. The apoptosis induced by SPR was associated with the activation of initiator caspases-8 and -9, as well as the effector caspase-3. SPR stimulated Bid cleavage, indicating that the apoptotic action of caspase-8-mediated Bid cleavage leads to the activation of caspase-9. SPR increased the expression of the pro-apoptotic protein, Bax, and decreased the expression of the anti-apoptotic protein, Bcl-2. SPR also increased the expression of the caspase-independent mitochondrial apoptosis factor, AIF, in HT-29 cells. These results indicate that SPR inhibits HT-29 cell proliferation by inducing apoptosis, which may be mediated via both caspase-dependent and -independent pathways. |
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Authors:
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Jae-Yong Kim; Kyoung-Wuk Park; Kwang-Deog Moon; Mi-Kyung Lee; Jina Choi; Sung-Tae Yee; Ki-Hwan Shim; Kwon-Il Seo |
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Publication Detail:
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Type: Journal Article Date: 2008-10-10 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 46 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 3753-8 Citation Subset: IM |
Affiliation:
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Department of Food Science and Technology, Kyungpook National University, Daegu 702-701, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspases / antagonists & inhibitors, metabolism Cell Proliferation / drug effects DNA Fragmentation / drug effects Enzyme Inhibitors / pharmacology HT29 Cells Humans L-Lactate Dehydrogenase / metabolism Mitochondria / drug effects Platycodon / chemistry* Saponins / isolation & purification, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Saponins; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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