Document Detail


Induction of apoptosis due to lowering the level of eukaryotic initiation factor 2-associated protein, p67, from mammalian cells by antisense approach.
MedLine Citation:
PMID:  9925753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p67, a cellular glycoprotein, protects eIF2alpha from phosphorylation by inhibitory kinases such as double-stranded RNA dependent eIF2 kinase, PKR, and heme-controlled repressor and thus promotes protein synthesis in mammalian cells. To investigate whether p67 is essential for the survival of mammalian cells, the basal level of p67 was lowered from rat tumor hepatoma cells using antisense approach. The antisense p67 RNA specifically lowered the levels of p67 message and the protein from these cells. As a result, the level of eIF2alpha phosphorylation increased significantly, the overall rate of protein synthesis decreased, and the rate of DNA synthesis also decreased in mammalian cells with low levels of p67 as compared to that seen in control cells. In addition, the majority of the cells with low levels of p67 are arrested at the G1 phase of the cell cycle and die with apoptosis. Taken together, these results suggest that appropriate levels of p67 is required for normal growth of mammalian cells.
Authors:
B Datta; R Datta
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  246     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-15     Completed Date:  1999-03-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  376-83     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
606 Hamilton Hall, University of Nebraska at Lincoln, Lincoln, Nebraska, 68588, USA. bdatta@unlinfo.unl.edu
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MeSH Terms
Descriptor/Qualifier:
Aminopeptidases*
Animals
Apoptosis*
Cell Cycle
Eukaryotic Initiation Factor-2 / metabolism*
Glycoproteins / genetics,  metabolism,  physiology*
Phosphorylation
RNA, Antisense*
Rats
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
GM22079/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Eukaryotic Initiation Factor-2; 0/Glycoproteins; 0/RNA, Antisense; EC 3.4.11.-/Aminopeptidases; EC 3.4.11.18/Metap2 protein, rat

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