| Induction of apoptosis and cell-cycle arrest in human colon cancer cells by meclizine. | |
| | |
MedLine Citation:
|
PMID: 17222494 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Meclizine (MEC), a histamine H1 antagonist, is used for the treatment of motion sickness and vertigo. In this study, we demonstrate that MEC dose-dependently induced apoptosis in human colon cancer cell lines (COLO 205 and HT 29 cells). Results of a DNA ladder assay revealed that DNA ladders appeared with MEC treatment in COLO 205 cells at dosage of >50 microM. In addition, the total cell number decreased dose-dependently after treatment with MEC in COLO 205 and HT 29 cells. Using flow cytometry, the percentage of COLO 205 cells arrested at G0/G1 phase increased dose-dependently. Analysis of changes in cell-cycle arrest-associated proteins with Western blotting showed that p53 and p21 were upregulated after treatment with MEC. The kinase activities of cyclin-dependent kinase 2 (CDK2) and CDK4 were suppressed in MEC-treated cells. As for apoptosis, MEC may induce upregulation of p53 and downregulation of Bcl-2, thus causing the release of cytochrome C from mitochondria and the translocation of apoptosis-inducing factor (AIF) to the nucleus. This resulted in the activation of caspase 3, 8, and 9. Our results provide the molecular basis of MEC-induced apoptosis and cell-cycle arrest in human colon cancer cells. |
| | |
Authors:
|
Jiunn-Chang Lin; Yuan-Soon Ho; Jie-Jen Lee; Chien-Liang Liu; Tsen-Long Yang; Chih-Hsiung Wu |
Related Documents
:
|
15761494 - Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53... 20354524 - Oncogenic kras sensitises colorectal tumour cells to chemotherapy by p53-dependent indu... 17266684 - Acid has antiproliferative effects in nonneoplastic barrett's epithelial cells. 9598874 - Therapeutic potential of recombinant p53 overexpression in breast cancer cells expressi... 7493644 - Uptake and metabolism of long-chain 1,2-diacylglycerols by rat adipocytes and h4iie hep... 45154 - Estimating the proportion of proliferating cells in a population. |
Publication Detail:
|
Type: Journal Article Date: 2006-12-09 |
Journal Detail:
|
Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 45 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2007 Jun |
Date Detail:
|
Created Date: 2007-04-06 Completed Date: 2007-07-24 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
|
Languages: eng Pagination: 935-44 Citation Subset: IM |
Affiliation:
|
Department of Surgery, Mackay Memorial Hospital, Taipei, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Apoptosis Inducing Factor / metabolism Blotting, Western Cell Cycle / drug effects* Cell Cycle Proteins / metabolism Cell Proliferation / drug effects Cell Survival / drug effects Colonic Neoplasms / drug therapy*, pathology Cyclin-Dependent Kinases / metabolism Cytochromes c / metabolism DNA Fragmentation / drug effects Dose-Response Relationship, Drug Flow Cytometry HT29 Cells Histamine H1 Antagonists / pharmacology Humans Meclizine / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Apoptosis Inducing Factor; 0/Cell Cycle Proteins; 0/Histamine H1 Antagonists; 569-65-3/Meclizine; 9007-43-6/Cytochromes c; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Selective hair analysis of nicotine by molecular imprinted solid-phase extraction: an application fo...
Next Document: Anandamide-induced Ca2+ elevation leading to p38 MAPK phosphorylation and subsequent cell death via ...