| Induction of apoptosis by the inhibitors of poly(ADP-ribose)polymerase in HeLa cells. | |
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MedLine Citation:
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PMID: 18695944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the role of poly(ADP-ribose)polymerase (PARP) in the physiological condition of cell growth, we studied the ability of PARP inhibitors to induce apoptosis. Benzamide (BA) and 4-amino-1,8-naphthalimide (NAP), two well-known inhibitors of PARP, treatment increased nuclear fragmentation and caspase-3 activity in HeLa (Human cervical cancer cell line) cells. The increase of cellular NAD(+) level was observed in HeLa cells treated with BA in comparison with untreated control cells. For unrevealing the specific PARP family member responsible for such induction of apoptosis we knocked down and over-expressed PARP-1 gene in HeLa cells. PARP-1 knock down cells were sensitive to BA induced nuclear fragmentation and caspase-3 activation while exogenous expression of PARP-1 rendered cells resistant to BA induced apoptosis. This result indicated that inhibition of PARP-1 resulted in induction of apoptosis. |
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Authors:
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Utpal Ghosh; Nitai P Bhattacharyya |
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Publication Detail:
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Type: Journal Article Date: 2008-08-10 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 320 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-10 Completed Date: 2009-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 15-23 Citation Subset: IM |
Affiliation:
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Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741235, India. utpal8ghosh@yahoo.co.in |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Naphthylamine
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analogs & derivatives*,
pharmacology Apoptosis / drug effects* Benzamides / pharmacology* Caspase 3 / metabolism Cell Nucleus / drug effects, metabolism Enzyme Activation Hela Cells* / drug effects, enzymology, physiology Humans Naphthalimides / pharmacology* Poly(ADP-ribose) Polymerases / antagonists & inhibitors*, genetics, metabolism Quinolones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 0/Naphthalimides; 0/Quinolones; 134-32-7/1-Naphthylamine; 1742-95-6/4-amino-1,8-naphthalimide; 27208-38-4/benzamide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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