| Induction of apoptosis by dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., in human skin melanoma cells. | |
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MedLine Citation:
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PMID: 15547680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dideoxypetrosynol A, a polyacetylene from the sponge Petrosia sp., is known to exhibit significant selective cytotoxicity against several human tumor cell lines. In the present study, we investigated the possible mechanisms by which dideoxypetrosynol A exerts its anti-proliferative action in cultured human SK-MEL-2 skin melanoma cells. Exposure of SK-MEL-2 cells to dideoxypetrosynol A resulted in growth inhibition and induction of apoptosis in a dose-dependent manner as measured by MTT assay, fluorescent microscopy and flow cytometry analysis. The increase in apoptosis was associated with a dose-dependent up-regulation in proapoptotic Bax expression and down-regulation of anti-apoptotic Bcl-2. Apoptosis-inducing concentrations of dideoxypetrosynol A induced caspase-3 and caspase-9 activation accompanied by proteolytic degradation of poly(ADP-ribose)-polymerase and selective down-regulation of cIAP-1. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of dideoxypetrosynol A. |
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Authors:
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Hye Joung Choi; Song-Ja Bae; Nam Deuk Kim; Jee H Jung; Yung Hyun Choi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 14 ISSN: 1107-3756 ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-19 Completed Date: 2005-04-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 1091-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Dongeui University College of Oriental Medicineand Biomedical Research Center of Oriental Medicine, Busan 614-052, Korea. choiyh@deu.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkynes
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chemistry,
pharmacology* Animals Apoptosis / drug effects* Caspases / metabolism Cell Line, Tumor Cell Proliferation / drug effects Down-Regulation / drug effects Enzyme Activation Fatty Alcohols / chemistry, pharmacology* Gene Expression Regulation, Neoplastic / drug effects Humans Inhibitor of Apoptosis Proteins Melanoma / pathology* Molecular Structure Petrosia / chemistry* Polyacetylenes Proteins / classification, metabolism Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Skin Neoplasms / pathology* bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Alkynes; 0/BAX protein, human; 0/Fatty Alcohols; 0/Inhibitor of Apoptosis Proteins; 0/Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 0/dideoxypetrosynol A; 25067-58-7/Polyacetylenes; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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