Document Detail


Induction of apoptosis by dexamethasone in the B cell lineage.
MedLine Citation:
PMID:  9776480     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The susceptibility to induction of apoptosis by the synthetic glucocorticoid, dexamethasone (Dex), was analysed at different stages of B cell maturation. Cells of the 70Z/3 pre-B cell line, expressing cytoplasmic mu chains, and LPS-stimulated 70Z/3 cells, expressing surface IgM, were used as a model of differentiation of pre-B cells into immature B cells. Cell proliferation and cell cycle progression were similarly inhibited by Dex (100 nM) in both naive 70Z/3 pre-B cells and in LPS-stimulated 70Z/3 cells. In contrast, Dex failed to affect apoptosis of naive 70Z/3 cells while it increased that of LPS-stimulated 70Z/3 cells. Splenic mature B lymphocytes were highly susceptible to Dex-induced apoptosis since subphysiological doses (5 nM) increased the frequency of apoptotic cells to more than 80%. On the other hand, the treatment of B lymphocytes with LPS, which led to proliferation and differentiation into immunoblasts, decreased the susceptibility to Dex-induced apoptosis. These effects were mediated by the glucocorticoid receptor since they were abrogated by the RU 486 antagonist. The response of B cells to glucocorticoids is thus dependent on their stage of differentiation.
Authors:
K Andréau; C Lemaire; V Souvannavong; A Adam
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunopharmacology     Volume:  40     ISSN:  0162-3109     ISO Abbreviation:  Immunopharmacology     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-12-17     Completed Date:  1998-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902474     Medline TA:  Immunopharmacology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
Affiliation:
Institut de Biochimie, CNRS ERS 0571, Université Paris-Sud, Orsay, France. karine.andreau@bbmpc.u-psud.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
B-Lymphocytes / drug effects*
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Line
Dexamethasone / pharmacology*
Female
Flow Cytometry
Glucocorticoids / pharmacology*
Lipopolysaccharides
Mice
Mice, Inbred C57BL
Spleen / cytology,  drug effects
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Lipopolysaccharides; 50-02-2/Dexamethasone

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