| Induction of apoptosis by beta-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 cells. | |
| | |
MedLine Citation:
|
PMID: 19420723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by beta-carotene and dimethyl tetrasulfide (Me(2)S(4)) assisted by UVA irradiation in HL-60 cells was assessed. beta-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me(2)S(4) combined with beta-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. beta-carotene was significantly degraded by UVA, but this was not accelerated by Me(2)S(4) in a cell culture system. The photodegradation products of beta-carotene prepared by UVA irradiation regardless of the addition of Me(2)S(4) showed lower cytotoxicity than beta-carotene itself in HL-60 cells. These results suggest that the ROS- and caspase-3-dependent apoptosis induced by beta-carotene and Me(2)S(4) assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of beta-carotene in HL-60 cells. |
| | |
Authors:
|
Gongliang Zhang; Haitao Wu; Beiwei Zhu; Yasuaki Shimoishi; Yoshimasa Nakamura; Yoshiyuki Murata |
Related Documents
:
|
10086973 - Cellular effects of beta-particle delivery on vascular smooth muscle cells and endothel... 7544273 - Recombinant thyrotropin containing a beta-subunit chimera with the human chorionic gona... 8846023 - Induction of a hyaluronan receptor, cd44, during embryonal carcinoma and embryonic stem... |
Publication Detail:
|
Type: Journal Article Date: 2009-05-07 |
Journal Detail:
|
Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-05-25 Completed Date: 2009-08-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
|
Languages: eng Pagination: 1014-20 Citation Subset: IM |
Affiliation:
|
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylcysteine
/
pharmacology Antineoplastic Agents / chemistry, pharmacology* Antioxidants / pharmacology Apoptosis / drug effects*, radiation effects* Caspase 3 / metabolism Cell Proliferation / drug effects, radiation effects Cell Survival / drug effects, radiation effects Enzyme Activation / drug effects, radiation effects HL-60 Cells Humans Jurkat Cells Photolysis / drug effects Reactive Oxygen Species / metabolism Sulfides / pharmacology* Ultraviolet Rays* beta Carotene / chemistry, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Antioxidants; 0/Reactive Oxygen Species; 0/Sulfides; 0/dimethyl tetrasulfide; 616-91-1/Acetylcysteine; 7235-40-7/beta Carotene; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Application of Trichoderma reesei cellulase and xylanase promoters through homologous recombination ...
Next Document: Efficient recovery of glucose and fructose via enzymatic saccharification of rice straw with soft ca...