Document Detail

Induction of apoptosis by beta-carotene and dimethyl tetrasulfide assisted by UVA irradiation in HL-60 cells.
MedLine Citation:
PMID:  19420723     Owner:  NLM     Status:  MEDLINE    
Cellular phototoxicity induced by UVA irradiation and its potential application to therapy has been reported. In the present study, the induction of apoptosis induced by beta-carotene and dimethyl tetrasulfide (Me(2)S(4)) assisted by UVA irradiation in HL-60 cells was assessed. beta-carotene assisted by UVA significantly decreased the cell viability and induced DNA fragmentation in HL-60 cells. Me(2)S(4) combined with beta-carotene and assisted by UVA significantly inhibited the cell viability, and enhanced the caspase-3 activity which was completely inhibited by N-acety-L-cysteine. beta-carotene was significantly degraded by UVA, but this was not accelerated by Me(2)S(4) in a cell culture system. The photodegradation products of beta-carotene prepared by UVA irradiation regardless of the addition of Me(2)S(4) showed lower cytotoxicity than beta-carotene itself in HL-60 cells. These results suggest that the ROS- and caspase-3-dependent apoptosis induced by beta-carotene and Me(2)S(4) assisted by UVA was due to a synergistic action rather than to the sole effect of the photodegradation products of beta-carotene in HL-60 cells.
Gongliang Zhang; Haitao Wu; Beiwei Zhu; Yasuaki Shimoishi; Yoshimasa Nakamura; Yoshiyuki Murata
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Publication Detail:
Type:  Journal Article     Date:  2009-05-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1014-20     Citation Subset:  IM    
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
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MeSH Terms
Acetylcysteine / pharmacology
Antineoplastic Agents / chemistry,  pharmacology*
Antioxidants / pharmacology
Apoptosis / drug effects*,  radiation effects*
Caspase 3 / metabolism
Cell Proliferation / drug effects,  radiation effects
Cell Survival / drug effects,  radiation effects
Enzyme Activation / drug effects,  radiation effects
HL-60 Cells
Jurkat Cells
Photolysis / drug effects
Reactive Oxygen Species / metabolism
Sulfides / pharmacology*
Ultraviolet Rays*
beta Carotene / chemistry,  pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Antioxidants; 0/Reactive Oxygen Species; 0/Sulfides; 0/dimethyl tetrasulfide; 616-91-1/Acetylcysteine; 7235-40-7/beta Carotene; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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