| Induction of apoptosis by benzamide and its inhibition by aurin tricarboxylic acid (ATA) in Chinese hamster V79 cells. | |
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MedLine Citation:
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PMID: 15450410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the effect of benzamide and nicotinamide, well known inhibitors of poly(ADP-ribose) polymerase, in Chinese hamster V79 cells at the physiological condition of cell growth, we have tested the ability of the inhibitors to induce apoptosis. Apoptosis was detected by nuclear fragmentation, nucleosomal ladder formation, cytochrome-c release from the mitochondria and caspase-3 activation. Benzamide treatment alone increased nuclear fragmentation in dose (2.5-10 mM) and time (4-48 h)-dependent manner. Such treatment also increased nucleosomal ladders. However, 5 mM benzamide pre-treatment inhibited the nucleosomal ladders induced by gamma-irradiation indicating the role of poly(ADP-ribose) polymerase was different in irradiated cells and in un-irradiated cells. Release of cytochrome-c from the mitochondria and caspase-3 activity were also increased by such treatment. Treatment with 200 microM of aurin tricarboxylic acid (ATA), an inhibitor of DNases, inhibited the nucleosomal ladders induced by benzamide or gamma-irradiation without changing the cytochrome-c release or caspase-3 activation. This result showed that ATA inhibited the nucleosomal ladders possibly by inhibiting DNase(s) involved in apoptosis. |
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Authors:
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Utpal Ghosh; Bhaswati Pandit; Jui Dutta; Nitai P Bhattacharyya |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 554 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-28 Completed Date: 2004-11-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 121-9 Citation Subset: IM |
Affiliation:
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Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Calcutta 700 064, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects*, radiation effects Aurintricarboxylic Acid / pharmacology* Benzamides / pharmacology* Blotting, Western Caspase 3 Caspases / metabolism Cell Line Cell Survival / drug effects Cricetinae Gamma Rays |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 27208-38-4/benzamide; 4431-00-9/Aurintricarboxylic Acid; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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