| Induction of apoptosis by the aqueous extract of Rubus coreanum in HT-29 human colon cancer cells. | |
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MedLine Citation:
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PMID: 16308138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The incompletely ripened fruit of Rubus coreanum (IRFRC) has been used in traditional herbal medicine to manage various diseases. To explore the possibility that IRFRC has chemopreventive effects, we examined whether or not extracts of IRFRC inhibits HT-29 cell growth and explored the mechanism for this effect. METHODS: We cultured HT-29 cells in the presence of the aqueous or ethanol extract of IRFRC. DNA synthesis was estimated by 5-bromo-2'-deoxyuridine incorporation. We measured apoptosis using a DNA fragmentation assay and Annexin V staining. We used western blot analyses to determine the cleavage of caspases and poly (adenosine diphosphate-ribose) polymerase. RESULTS: Aqueous extract of IRFRC substantially inhibited viable HT-29 cell number in a dose-dependent manner, whereas ethanol extract had only a minimal effect. Aqueous extract inhibited DNA synthesis and induced apoptosis of HT-29 cells in a dose-dependent manner. Aqueous extract induced cleavage of caspase-3, -7, and -9 and induced the activity of caspase-3 and cleavage of poly (adenosine diphosphate-ribose) polymerase. CONCLUSIONS: We have shown that aqueous extract of IRFRC inhibits cell proliferation and stimulates apoptosis in HT-29 cells, and that this may be mediated by its ability to activate the caspase-3 pathway. It remains to be determined whether the aqueous extract of IRFRC has chemopreventive activities in animal models. |
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Authors:
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Eun Ji Kim; Yong-Jin Lee; Hyun-Kyung Shin; Jung Han Yoon Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 21 ISSN: 0899-9007 ISO Abbreviation: Nutrition Publication Date: 2005 Nov-Dec |
Date Detail:
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Created Date: 2005-11-25 Completed Date: 2006-03-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 1141-8 Citation Subset: IM |
Affiliation:
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Silver Biotechnology Research Center, Hallym University, Chuncheon, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology* Apoptosis / drug effects* Blotting, Western Caspase 3 Caspases / metabolism Cell Division / drug effects Colonic Neoplasms / pathology, prevention & control* DNA Fragmentation Dose-Response Relationship, Drug Fruit / chemistry* HT29 Cells Humans Plant Extracts / pharmacology* Poly(ADP-ribose) Polymerases / metabolism Rosaceae / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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