| Induction of apoptosis by (Z)-stellettic acid C, an acetylenic acid from the sponge Stelletta sp., is associated with inhibition of telomerase activity in human leukemic U937 cells. | |
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MedLine Citation:
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PMID: 17356266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: (Z)-stellettic acid C, an acetylenic acid from the marine sponge Stelletta sp., has been shown to have cytotoxic activity in some cancer cells; however, its mechanisms on malignant cell growth are not known. In this study, the potential of (Z)-stellettic acid C to induce apoptosis in human leukemic U937 cells and its effects on telomerase activity were investigated. METHODS: Cytotoxicity was evaluated by MTT assays. Apoptosis was detected using DAPI staining and annexin V fluorescein. The mRNAs of Bcl-2, inhibitor of apoptosis proteins (IAPs) family and Fas/FasL system were determined by RT-PCR. Caspases and telomerase activities were measured using colorimetric assay and telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA), respectively. RESULTS: Exposure of U937 cells to (Z)-stellettic acid C resulted in growth inhibition and induction of apoptosis in a dose-dependent manner, which was associated with the modulation of Bcl-2 family expression, activation of caspases and downregulation of IAPs family members. (Z)-Stellettic acid C treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase, a main determinant of the telomerase enzymatic activity, was progressively downregulated by (Z)-stellettic acid C treatment. CONCLUSIONS: These results suggest that (Z)-stellettic acid C could have a possible cancer therapeutic potential. |
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Authors:
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Cheol Park; Gi-Young Kim; Won Il Kim; Sang Hoon Hong; Dong Il Park; Nam Deuk Kim; Song-Ja Bae; Jee H Jung; Yung Hyun Choi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-15 |
Journal Detail:
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Title: Chemotherapy Volume: 53 ISSN: 1421-9794 ISO Abbreviation: Chemotherapy Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-11 Completed Date: 2007-05-01 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0144731 Medline TA: Chemotherapy Country: Switzerland |
Other Details:
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Languages: eng Pagination: 160-8 Citation Subset: IM |
Copyright Information:
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Copyright 2007 S. Karger AG, Basel. |
Affiliation:
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Department of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkynes
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pharmacology* Animals Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Apoptosis Regulatory Proteins / genetics Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Proliferation / drug effects Fatty Acids, Unsaturated / pharmacology* Gene Expression Regulation, Neoplastic / drug effects Humans Microtubule-Associated Proteins / genetics Neoplasm Proteins / genetics Porifera / chemistry* RNA, Messenger / metabolism Telomerase / antagonists & inhibitors*, genetics, metabolism U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Alkynes; 0/Antineoplastic Agents; 0/Apoptosis Regulatory Proteins; 0/BIRC5 protein, human; 0/Fatty Acids, Unsaturated; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/RNA, Messenger; 2834-03-9/2-hexadecynoic acid; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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