Document Detail


Induction of apoptosis by (Z)-stellettic acid C, an acetylenic acid from the sponge Stelletta sp., is associated with inhibition of telomerase activity in human leukemic U937 cells.
MedLine Citation:
PMID:  17356266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: (Z)-stellettic acid C, an acetylenic acid from the marine sponge Stelletta sp., has been shown to have cytotoxic activity in some cancer cells; however, its mechanisms on malignant cell growth are not known. In this study, the potential of (Z)-stellettic acid C to induce apoptosis in human leukemic U937 cells and its effects on telomerase activity were investigated. METHODS: Cytotoxicity was evaluated by MTT assays. Apoptosis was detected using DAPI staining and annexin V fluorescein. The mRNAs of Bcl-2, inhibitor of apoptosis proteins (IAPs) family and Fas/FasL system were determined by RT-PCR. Caspases and telomerase activities were measured using colorimetric assay and telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA), respectively. RESULTS: Exposure of U937 cells to (Z)-stellettic acid C resulted in growth inhibition and induction of apoptosis in a dose-dependent manner, which was associated with the modulation of Bcl-2 family expression, activation of caspases and downregulation of IAPs family members. (Z)-Stellettic acid C treatment markedly inhibited the activity of telomerase in a dose-dependent fashion. Additionally, the expression of human telomerase reverse transcriptase, a main determinant of the telomerase enzymatic activity, was progressively downregulated by (Z)-stellettic acid C treatment. CONCLUSIONS: These results suggest that (Z)-stellettic acid C could have a possible cancer therapeutic potential.
Authors:
Cheol Park; Gi-Young Kim; Won Il Kim; Sang Hoon Hong; Dong Il Park; Nam Deuk Kim; Song-Ja Bae; Jee H Jung; Yung Hyun Choi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-15
Journal Detail:
Title:  Chemotherapy     Volume:  53     ISSN:  1421-9794     ISO Abbreviation:  Chemotherapy     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-11     Completed Date:  2007-05-01     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0144731     Medline TA:  Chemotherapy     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  160-8     Citation Subset:  IM    
Copyright Information:
Copyright 2007 S. Karger AG, Basel.
Affiliation:
Department of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan, Korea.
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MeSH Terms
Descriptor/Qualifier:
Alkynes / pharmacology*
Animals
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Apoptosis Regulatory Proteins / genetics
Caspase 3 / metabolism
Caspase 8 / metabolism
Caspase 9 / metabolism
Cell Proliferation / drug effects
Fatty Acids, Unsaturated / pharmacology*
Gene Expression Regulation, Neoplastic / drug effects
Humans
Microtubule-Associated Proteins / genetics
Neoplasm Proteins / genetics
Porifera / chemistry*
RNA, Messenger / metabolism
Telomerase / antagonists & inhibitors*,  genetics,  metabolism
U937 Cells
Chemical
Reg. No./Substance:
0/Alkynes; 0/Antineoplastic Agents; 0/Apoptosis Regulatory Proteins; 0/BIRC5 protein, human; 0/Fatty Acids, Unsaturated; 0/Microtubule-Associated Proteins; 0/Neoplasm Proteins; 0/RNA, Messenger; 2834-03-9/2-hexadecynoic acid; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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