| Induction of apoptosis by Hax-1 siRNA in melanoma cells. | |
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MedLine Citation:
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PMID: 19254774 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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HS1-associated protein X-1 (Hax-1) is a novel intracellular protein and recent studies suggested that it is an anti-apoptotic factor in different tumors. Hax-1 expression was upregulated in various metastatic tumors and cancer cell lines, including melanoma. To understand the role of Hax-1 in melanoma development and progression, we constructed Hax-1 short interfering RNA (siRNA) expression vectors to downregulate Hax-1 expression in a human melanoma A375 cell line. One of the two Hax-1 RNA interference (RNAi) constructs significantly reduced melanoma cell viability, which was due to induction of apoptosis in A375 cells. Molecularly, the induced apoptosis through downregulation of Hax-1 expression was mediated by activation of caspase-3 and poly-ADP-ribose polymerase (PARP) enzymatic activity in A375 cells. The data indicate that Hax-1 plays a role in suppression of apoptosis and promotion of melanoma cell growth, suggesting that this Hax-1 siRNA has a therapeutic indication in control of melanoma. |
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Authors:
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Wen-Bin Li; Jie Feng; Song-Mei Geng; Peng-Yu Zhang; Xiao-Ning Yan; Gang Hu; Cai-Qing Zhang; Bing-Jun Shi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-28 |
Journal Detail:
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Title: Cell biology international Volume: 33 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-06-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: Netherlands |
Other Details:
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Languages: eng Pagination: 548-54 Citation Subset: IM |
Affiliation:
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Department of Dermatology, The Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, The West Five Road #157, Xi'an, Shaanxi, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Caspase 3 / metabolism Cell Line, Tumor Cell Survival / physiology Down-Regulation / physiology Gene Knockdown Techniques Humans Melanoma / genetics*, pathology*, therapy Poly(ADP-ribose) Polymerases / metabolism Proteins / antagonists & inhibitors*, genetics RNA, Small Interfering / genetics |
| Chemical | |
Reg. No./Substance:
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0/HAX1 protein, human; 0/Proteins; 0/RNA, Small Interfering; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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