Document Detail


Induction of anoikis by sodium arsenite in rat hepatoma FGC4 cells: implications for assessment of regulation of heat shock protein 70.
MedLine Citation:
PMID:  22827573     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Context: Arsenic, a toxic metal with major health concerns, elicits up-regulation of heat shock protein 70 (HSP70) in rat hepatoma FGC4 cells, together with evidence of detachment of viable cells from the growth substratum. Objective: To determine if this cell detachment was linked to anoikis, and the impact of this on measurement of HSP70 expression. Materials and Methods: FGC4 cells were exposed to sodium arsenite, and detached and attached cells were taken for assessment of cell viability, activation of pro-caspase 3, and expression of HSP70. Results: Exposure to sodium arsenite led to loss of viable cells from the substratum, associated with apoptosis in detached, but not attached, cells. Up-regulation of HSP70 of a similar magnitude was demonstrated in both cell populations. Exposure of cells to cadmium chloride, another toxic metal believed to act by an oxidative stress mechanism, produced very little release of viable cells from the culture substratum, was not associated with apoptosis, but did elicit a modest up-regulation of HSP70 in both cell populations. Discussion: Exposure of FGC4 cells to sodium arsenite elicits anoikis, a form of anchorage-dependent apoptosis, and assessment of the level of HSP70 up-regulation in such cells should take account of the detached cell population. Further, the data suggest that this phenomenon is selective to arsenic, rather than to another metal that shares a similar mechanism of toxicity.
Authors:
Mudafara Bengleil; Sherifa Hassaneen; Jeffrey R Fry
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-24
Journal Detail:
Title:  Toxicology mechanisms and methods     Volume:  -     ISSN:  1537-6524     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134521     Medline TA:  Toxicol Mech Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK.
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