Document Detail


Induction of alveolar epithelial injury by phospholipase A2.
MedLine Citation:
PMID:  2917929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Severe damage to the alveolar type I epithelial cell is a characteristic morphological feature of lung injury due to numerous cases. It is postulated that excess phospholipase A2 (PLA2) activity might be responsible for these changes, as one of the naturally occurring products of this enzyme, lysophosphatidylcholine (lysoPC) has been shown to cause selective injury to the type I pneumonocyte when it is instilled into the lower air spaces of the lung. To further investigate this potential mechanism of type I epithelial cell toxicity, we have measured the epithelial permeability-surface area product (PS) for [14C]sucrose as well as whole-lung lysoPC content at several times after instilling PLA2 (Naja naja venom) into either the air spaces or the perfusate of an isolated hamster lung preparation. As a molar percentage of total phospholipids, the normal hamster lung contains approximately 1.5% lysoPC, and this value is not affected by fluid filling of the air spaces or perfusion of the excised lung for periods up to 90 min. When 0.15 U/ml PLA2 is instilled into the air spaces, lung lysoPC content increases to approximately 2.5% and there are barely detectable increases in [14C]sucrose PS. With air space PLA2 concentrations of 0.30 U/ml, lysoPC content increases to between 4 and 5%, [14C]sucrose PS increases by greater than a factor of 10, and flooding of the alveolar spaces occur. Ultrastructural studies of similarly treated lungs show widespread but selective damage to the type I epithelial cells. These same biochemical and functional changes are not seen when the same concentrations of PLA2 are added to the lung perfusate.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
D E Niewoehner; K Rice; P Duane; A A Sinha; R Gebhard; D Wangensteen
Related Documents :
18657059 - Reduced lung uptake of iodine-123 metaiodobenzylguanidine in high-altitude pulmonary oe...
1011959 - The effects of physical training on the lung growth of infant rats.
2601619 - Fulminant hyperkalaemia and multiple complications following ibuprofen overdose.
22556019 - Cerebral cortex oxygen delivery and exercise limitation in patients with copd.
7338819 - The summation of left and right lung volume information in the control of breathing in ...
15477499 - Hypercapnia via reduced rate and tidal volume contributes to lipopolysaccharide-induced...
24815919 - Insulinogenic sucrose+amino acid mixture ingestion immediately after resistance exercis...
22114179 - Influence of rest and exercise at a simulated altitude of 4,000 m on appetite, energy i...
3308809 - Altitude acclimatization attenuates plasma ammonia accumulation during submaximal exerc...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  66     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  261-7     Citation Subset:  IM    
Affiliation:
Veterans Administration Medical Center, Minneapolis, Minnesota.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bronchoalveolar Lavage Fluid / metabolism
Cricetinae
Epithelium / metabolism
Female
Hydrolysis
Lung / drug effects,  metabolism,  physiology
Lysophosphatidylcholines / metabolism
Mesocricetus
Microscopy, Electron
Perfusion
Permeability
Phosphatidylcholines / metabolism
Phospholipases / pharmacology*
Phospholipases A / pharmacology*
Phospholipases A2
Phospholipids / metabolism
Pulmonary Alveoli / drug effects*,  pathology
Chemical
Reg. No./Substance:
0/Lysophosphatidylcholines; 0/Phosphatidylcholines; 0/Phospholipids; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Is resting muscle oxygen uptake controlled by oxygen availability to cells?
Next Document:  Transmission of sound generated by sternal percussion.