| Induction of adrenomedullin by hypoxia in cultured retinal pigment epithelial cells. | |
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MedLine Citation:
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PMID: 11274089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To explore the effects of hypoxia on the production and secretion of adrenomedullin (ADM) and endothelin (ET)-1 in human retinal pigment epithelial (RPE) cells. METHODS: RPE cells were cultured under normoxic or hypoxic (1% O2) conditions. Expression of ADM and ET-1 was examined by Northern blot analysis and radioimmunoassay. Effects of ADM and ET-1 on the number of RPE cells were examined by modified 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: ADM mRNA expression levels and immunoreactive ADM levels in the medium were increased by hypoxia in all three human RPE cell lines (ARPE-19, D407, and F-0202). Immunoreactive ET was detected in the cultured media of D407 cells and ARPE-19 cells and identified as ET-1 by reversed-phase high performance liquid chromatography. Hypoxia treatment for 48 hours increased immunoreactive ET levels approximately 1.3-fold in the cultured media of D407, but not ARPE-19 cells. Hypoxia decreased the number of ARPE-19 cells and F-0202 cells, and the treatment with ADM ameliorated the hypoxia-induced decrease in the cell number. In contrast, exogenously added ET-1 had no significant effects on the number of ARPE-19 cells under normoxia and hypoxia. CONCLUSIONS: Hypoxia increased the expression of ADM in all three human RPE cell lines, whereas the induction of ET-1 by hypoxia was found only in D407 cells. ADM induced by hypoxia may have protective roles against hypoxic cell damage in RPE cells. |
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Authors:
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T Udono; K Takahashi; M Nakayama; A Yoshinoya; K Totsune; O Murakami; Y K Durlu; M Tamai; S Shibahara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 42 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-03-29 Completed Date: 2001-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1080-6 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Miyagi, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenomedullin Anoxia / metabolism* Blotting, Northern Cell Count Cells, Cultured Chromatography, High Pressure Liquid Endothelin-1 / biosynthesis, genetics, pharmacology Humans Peptides / genetics, metabolism*, pharmacology Pigment Epithelium of Eye / cytology, drug effects, metabolism* RNA, Messenger / biosynthesis Radioimmunoassay Vasodilator Agents / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Peptides; 0/RNA, Messenger; 0/Vasodilator Agents; 148498-78-6/Adrenomedullin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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