Document Detail

Induction of activated caspase-3-immunoreactivity and apoptosis in the trigeminal ganglion neurons by neonatal peripheral nerve injury.
MedLine Citation:
PMID:  15261121     Owner:  NLM     Status:  MEDLINE    
Immunohistochemistry for activated caspase-3 and terminal deoxynucleotidyl transferease-mediated dUTP-biotin nick end labeling (TUNEL) was performed on the trigeminal ganglion after infraorbital nerve transection in newborn rats. The injury induced caspase-3-immunoreactivity and DNA fragmentation in neuronal cell bodies in the maxillary division of the ganglion ipsilateral to the injury. Starting at 16 h post-injury the immunoreactive and TUNEL-positive neurons increased and reached the peak at 24 h (7.9% and 8.9%, respectively). Thereafter they decreased and returned to the normal control level (<<1%) by 72 h. A double staining procedure revealed coexpression of caspase-3-immunoreactivity and DNA fragmentation. 75.5% (114/151) of TUNEL-positive neurons expressed the immunoreactivity, while 84.4% (114/135) of immunoreactive neurons exhibited DNA fragmentation signal. These results suggest that caspase-3 plays an important role in apoptotic elimination of neonatally axotomized rodent primary neurons.
Tomosada Sugimoto; Haiwei Jin; Masako Fijita; Tomohiro Fukunaga; Noriyuki Nagaoka; Tomoichiro Yamaai; Hiroyuki Ichikawa
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Brain research     Volume:  1017     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-20     Completed Date:  2004-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  238-43     Citation Subset:  IM    
Department of Oral Function and Anatomy, Okayama University, Graduate School of Medicine and Dentistry, Japan.
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MeSH Terms
Animals, Newborn
Apoptosis / physiology*
Caspase 3
Caspases / metabolism*
DNA Fragmentation
Enzyme Activation
Immunohistochemistry / methods
In Situ Nick-End Labeling / methods
Neurons / cytology*,  enzymology
Orbit / innervation*
Rats, Sprague-Dawley
Time Factors
Trigeminal Ganglion / cytology*,  metabolism
Reg. No./Substance:
EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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