Document Detail

Induction of the acrosome reaction in goat spermatozoa in simple physiological salt solution.
MedLine Citation:
PMID:  2760579     Owner:  NLM     Status:  MEDLINE    
The present study was carried out to determine whether K+, Mg2+, PO4(3-), and HCO3- in a medium are necessary for inducing the acrosome reaction in ejaculated goat spermatozoa. Washed goat spermatozoa were resuspended in K-1 medium, containing NaCl, KCl, CaCl2, MgCl2, NaH2PO4, and NaHCO3; in K-2 medium, containing NaCl, CaCl2, NaH2PO4, and NaHCO3; in K-3 medium, containing NaCl, CaCl2, and NaHCO3; and in K-4 medium, containing only NaCl and CaCl2, followed by preincubation in a sealed glass tube at 39.5 degrees C for 1, 2, or 3 h. The sperm acrosome reaction was evaluated by the trypan blue-Giemsa method and hamster test. The results were essentially the same in all cases. Following preincubation for 1 h, however, the percentage of acrosome-reacted spermatozoa, the proportion of zona-free hamster eggs penetrated by spermatozoa, and the average number of spermatozoa in the vitellus of these penetrated eggs were low; all values indicated a significant increase with preincubation for 2 and 3 h. The presence of K+, Mg2+, PO4(3-), and HCO3- in the medium thus does not appear necessary for inducing the acrosome reaction in goat spermatozoa, since they can undergo the reaction during preincubation in a simple physiological salt solution containing only NaCl and CaCl2 when preincubated in sealed glass tubes at 39.5 degrees C.
H Kusunoki; S Kato; S Kanda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  250     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-09-12     Completed Date:  1989-09-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  346-8     Citation Subset:  IM    
Graduate School of Science and Technology, Kobe University, Japan.
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MeSH Terms
Acrosome / drug effects,  physiology*
Bicarbonates / pharmacology*
Culture Media
Goats / physiology*
Magnesium / pharmacology
Phosphates / pharmacology
Potassium / pharmacology
Spermatozoa / drug effects,  physiology*
Reg. No./Substance:
0/Bicarbonates; 0/Culture Media; 0/Phosphates; 7439-95-4/Magnesium; 7440-09-7/Potassium

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