|Induction of Th1-type immune response by chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen Der p 2 for oral vaccination in mice.|
|PMID: 19254479 Owner: NLM Status: MEDLINE|
|This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles, suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a higher level expression of gene in vivo. Oral chitosan-pDer p 2 nanoparticles in BALB/c mice can induce IFN-gamma in serum and prevent subsequent sensitization of Th2 cell-regulated specific IgE responses. The data indicate that the oral administration of chitosan-pDer p 2 nanoparticles results in the expression of Der p 2 in the epithelial cells of both stomach and small intestine and the induction of Th1-type immune response.|
|Guo Ping Li; Zhi Gang Liu; Bin Liao; Nan Shan Zhong|
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|Type: Journal Article|
|Title: Cellular & molecular immunology Volume: 6 ISSN: 2042-0226 ISO Abbreviation: Cell. Mol. Immunol. Publication Date: 2009 Feb|
|Created Date: 2009-03-03 Completed Date: 2009-05-12 Revised Date: 2014-09-20|
Medline Journal Info:
|Nlm Unique ID: 101242872 Medline TA: Cell Mol Immunol Country: China|
|Languages: eng Pagination: 45-50 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Allergens / genetics, immunology
Antigens, Dermatophagoides / genetics, immunology*
Chitosan / administration & dosage, immunology
Hypersensitivity / therapy*
Immunoglobulin E / blood
Interferon-gamma / blood
Interleukin-4 / blood
Intestine, Small / immunology
Mice, Inbred BALB C
Nanoparticles / administration & dosage
Plasmids / administration & dosage, immunology
Stomach / immunology
Th1 Cells / immunology*
Vaccines, DNA / genetics, immunology*, therapeutic use
|0/Allergens; 0/Antigens, Dermatophagoides; 0/Arthropod Proteins; 0/Dermatophagoides pteronyssinus antigen p 2; 0/Vaccines, DNA; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma; 9012-76-4/Chitosan|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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