Document Detail

Induction of Th1-type immune response by chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen Der p 2 for oral vaccination in mice.
MedLine Citation:
PMID:  19254479     Owner:  NLM     Status:  MEDLINE    
This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles, suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a higher level expression of gene in vivo. Oral chitosan-pDer p 2 nanoparticles in BALB/c mice can induce IFN-gamma in serum and prevent subsequent sensitization of Th2 cell-regulated specific IgE responses. The data indicate that the oral administration of chitosan-pDer p 2 nanoparticles results in the expression of Der p 2 in the epithelial cells of both stomach and small intestine and the induction of Th1-type immune response.
Guo Ping Li; Zhi Gang Liu; Bin Liao; Nan Shan Zhong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular & molecular immunology     Volume:  6     ISSN:  2042-0226     ISO Abbreviation:  Cell. Mol. Immunol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-03-03     Completed Date:  2009-05-12     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  101242872     Medline TA:  Cell Mol Immunol     Country:  China    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
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MeSH Terms
Administration, Oral
Allergens / genetics,  immunology
Antigens, Dermatophagoides / genetics,  immunology*
Arthropod Proteins
Chitosan / administration & dosage,  immunology
Gene Expression
Genetic Therapy*
HeLa Cells
Hypersensitivity / therapy*
Immunoglobulin E / blood
Interferon-gamma / blood
Interleukin-4 / blood
Intestine, Small / immunology
Mice, Inbred BALB C
Nanoparticles / administration & dosage
Plasmids / administration & dosage,  immunology
Stomach / immunology
Th1 Cells / immunology*
Vaccines, DNA / genetics,  immunology*,  therapeutic use
Reg. No./Substance:
0/Allergens; 0/Antigens, Dermatophagoides; 0/Arthropod Proteins; 0/Dermatophagoides pteronyssinus antigen p 2; 0/Vaccines, DNA; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 82115-62-6/Interferon-gamma; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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