Document Detail

Induction of Th1 polarized immune responses by thiolated Eudragit-coated F4 and F18 fimbriae of enterotoxigenic Escherichia coli.
MedLine Citation:
PMID:  21571066     Owner:  NLM     Status:  Publisher    
Diarrhea in newborn and weaned piglets is mainly induced by enterotoxigenic Escherichia coli (ETEC) with fimbriae F4 (K88) and F18 (F107). In this study, we evaluated F4 and F18 coated with thiolated Eudragit microspheres (TEMS) as a candidate for an oral vaccine. The average particle sizes of TEMS, F4-loaded TEMS, and F18-loaded TEMS were measured as 4.2±0.75μm, 4.7±0.50μm, and 4.5±0.37μm, respectively. F4 is more efficiently encapsulated than F18 in the loading with TEMS. In the release test, F4 and F18 fimbriae were protected in acidic circumstances, whereas most were released at pH 7.4 of intestine circumstances. Production of TNF-α and NO from RAW 264.7cells was increased in a time-dependent manner after exposure to all groups, whereas only F4- or F18-loaded TEMS-stimulated IL-6 secretion. The levels of IFN-γ from mouse splenocytes after exposure to F4 or F18 were increased while IL-4 was not detectable. These results suggest that F4- and F18-loaded TEMS may effectively induce immune response with the efficient release of antigens to appropriate target sites.
Won-Jung Lee; Seungbin Cha; Minkyoung Shin; Mohammad Ariful Islam; Chong-Su Cho; Han Sang Yoo
Related Documents :
21992896 - Salvianolic acid b suppresses ifn-γ-induced jak/stat1 activation in endothelial cells.
21803656 - Nlrs in immune privileged sites.
22208616 - The nuclear factor-κb inhibitor pyrrolidine dithiocarbamate reduces polyinosinic-polyc...
22179576 - Hsp27 silencing coordinately inhibits proliferation and promotes fas-induced apoptosis ...
20027136 - Cyanohepatotoxins influence on the neuroendocrine and immune systems in fish - a short ...
22937496 - Nucleotide oligomerization domain 2 contributes to the innate immune response in thce c...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-6
Journal Detail:
Title:  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V     Volume:  -     ISSN:  1873-3441     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9109778     Medline TA:  Eur J Pharm Biopharm     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Department of Infectious Diseases, College of Veterinary Medicine, Seoul, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Increase in CpG DNA-induced inflammatory responses by DNA oxidation in macrophages and mice.
Next Document:  Diuretic bioactivity optimization of furosemide in rats.