| Induction of an oxalate decarboxylase in the filamentous fungus Trametes versicolor by addition of inorganic acids. | |
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MedLine Citation:
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PMID: 19266319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to improve yields and to reduce the cost of oxalate decarboxylase (OxDC, EC 4.1.1.2), the induction of OxDC in the white-rot fungus Trametes versicolor was studied in this work. OxDC was induced by addition of inorganic acids including hydrochloric acid, sulfuric acid, and phosphoric acid to culture media. The results showed that all the acids could enhance OxDC expression. The activity of the acid-induced OxDC rose continuously. All of the OxDC volumetric activities induced by the inorganic acids were higher than 20.0 U/L and were two times higher than that obtained with oxalic acid. OxDC productivity was around 4.0 U*L(-1)*day(-1). The highest specific activity against total protein was 3.2 U/mg protein at day 8 after induction of sulfuric acid, and the specific activity against mycelial dry weight was 10.6 U/g at day 9 after induction of hydrochloric acid. The growth of mycelia was inhibited slightly when the pH values in culture media was around 2.5-3.0, while the growth was inhibited heavily when the pH was lower than 2.5. |
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Authors:
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Cui Xia Zhu; Feng Hong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-06 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 160 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-26 Completed Date: 2010-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 655-64 Citation Subset: IM |
Affiliation:
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Donghua University, Shanghai, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids
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metabolism* Carboxy-Lyases / metabolism* Fungal Proteins / metabolism* Mycelium / growth & development Trametes / enzymology*, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Fungal Proteins; EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.2/oxalate decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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