| Induction of NO synthase 2 in ventricular cardiomyocytes incubated with a conventional bicarbonate dialysis bath. | |
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MedLine Citation:
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PMID: 18281316 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In hypotension-prone patients, conventional bicarbonate dialysis (BD) causes a reduced cardiovascular tolerance to the treatment with respect to acetate-free biofiltration (AFB). One possible explanation is an overproduction of endogenous NO (nitric oxide) due to the residual quote of acetate (4 mM) in the BD dialysate formulation. NO overload might cause the impairment of cardiovascular reactivity observed during BD. In this study, a potential direct impact of the residual quote of acetate on the cardiac cells was investigated. METHODS: Ventricular cardiac myocytes isolated from adult rat hearts were treated with three different dialysis baths with or without acetate: BD, AFB and AFB + 4 mM of acetate (AFB(+)). Corresponding levels of expression of the inducible NO synthase 2 (NOS2) were assessed after the treatments along with the measurement of single-cell action potential (AP). RESULTS: Incubation with acetate-containing dialysis solutions significantly enhanced (P < 0.05) the expression of NOS2 mRNA (BD: 1.11 +/- 0.31; AFB(+): 0.73 +/- 0.04, NOS2/GAPDH intensitometric ratio) with respect to the acetate-free bath (AFB: 0.03 +/- 0.01). Accordingly, protein translation was also enhanced (BD: 0.176 +/- 0.021; AFB(+): 0.135 +/- 0.009, NOS2/alpha-tubuline intensitometric ratio) with respect to AFB (0.002 +/- 0.001, P < 0.05). Measurement of the AP indicates that acetate-containing solutions determine a shortening of the repolarization phase as compared to treatment with AFB (BD: 95 +/- 13; AFB(+): 76 +/- 10; AFB: 162 +/- 16 ms). CONCLUSION: These findings show that the residual quote of acetate of the BD bath formulation affects the expression of NOS2 and the duration of AP in cardiac cells. This might cause the cardiac contractile impairment in unstable patients during BD treatment. |
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Authors:
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Eleonora Grandi; Marco Govoni; Simone Furini; Stefano Severi; Emanuele Giordano; Antonio Santoro; Silvio Cavalcanti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-14 |
Journal Detail:
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Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 23 ISSN: 1460-2385 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-30 Completed Date: 2008-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: England |
Other Details:
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Languages: eng Pagination: 2192-7 Citation Subset: IM |
Affiliation:
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Laboratory of Cellular and Molecular Engineering, University of Bologna, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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pharmacology Action Potentials / drug effects Animals Bicarbonates / pharmacology* Dialysis Solutions / pharmacology* Heart Ventricles / cytology, enzymology* Hemodiafiltration Male Myocardial Contraction / drug effects Myocytes, Cardiac / cytology, enzymology* Nitric Oxide Synthase Type II / metabolism* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Bicarbonates; 0/Dialysis Solutions; 0/RNA, Messenger; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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