Document Detail

Induction of M-phase arrest and apoptosis after HIV-1 Vpr expression through uncoupling of nuclear and centrosomal cycle in HeLa cells.
MedLine Citation:
PMID:  10896777     Owner:  NLM     Status:  MEDLINE    
The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpr induces cell cycle arrest in the G2 phase of the cell cycle followed by apoptosis. The mechanism of the arrest is unknown but the arrest is believed to facilitate viral replication. In the present study, we have established cell lines that allow conditional expression of Vpr, and have examined the mechanism of cell death following Vpr expression. We found that cells expressing Vpr enter M phase after long G2 arrest but formed aberrant multipolar spindles that were incapable of completing karyokinesis or cytokinesis. This abnormality provided the basis for apoptosis, which always followed in these cells. The multipolar spindles formed in response to abnormal centrosomal duplication that occurred during the G2 arrest but did not occur in cells arrested in G2 by irradiation. Thus, the expression of Vpr appears to be responsible for abnormal centrosome duplication, which in turn contributes in part to the rapid cell death following HIV-1 infection.
N Watanabe; T Yamaguchi; Y Akimoto; J B Rattner; H Hirano; H Nakauchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  258     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-22     Completed Date:  2000-08-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  261-9     Citation Subset:  IM; X    
Copyright Information:
Copyright 2000 Academic Press.
Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
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MeSH Terms
Cell Division
Cell Line, Transformed
Cell Nucleus / virology
Centrosome / virology
G2 Phase
Gene Products, vpr / biosynthesis,  genetics,  physiology*
HIV-1 / physiology*
Hela Cells
Recombinant Fusion Proteins / biosynthesis,  genetics
vpr Gene Products, Human Immunodeficiency Virus
Reg. No./Substance:
0/Gene Products, vpr; 0/Recombinant Fusion Proteins; 0/vpr Gene Products, Human Immunodeficiency Virus

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