| Induction of Luciferase Activity under the Control of an hsp70 Promoter Using High-Intensity Focused Ultrasound: Combination of Bioluminescence and MRI Imaging in Three Different Tumour Models. | |
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MedLine Citation:
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PMID: 21381798 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The in vivo temporal changes of luciferase activity were investigated under the control of an hsp70 promoter in three tumour models after the application of different intensities of high-intensity focused ultrasound (HIFU). Three cell lines, SCCVII, NIH3T3 and M21 were stably transfected with a plasmid containing the hsp70 promoter and luciferase reporter gene, and tumours were subcutaneously initiated into mice. At a size of 1300 ± 234 mm(3), the tumours were exposed to five intensities of continuous HIFU (802-1401-2157-3067-4133 W/cm(2)) for 20 sec. Bioluminescence and MR imaging were performed to assess luciferase activity and signal intensity changes in the tissue. The MRI scan protocol was pre- and post-contrast T1-wt-SE, T2-wt-FSE, DCE-MRI, diffusion-wt STEAM sequence, T2 relaxation time determination obtained on a 1.5-T GE MRI scanner. The NIH3T3 tumours showed the highest luciferase activity of 328.1 ± 7.1 fold at 24 h at a HIFU intensity of 3067 W/cm(2), the M21 tumours of 3.2 ± 0.6 fold 8 hours and the SCCVII tumours 2.9 ± 0.9 fold 4 hours post-HIFU at 2157 W/cm(2). The greatest increase in T2 signal intensity and T2 relaxation time of 20.7 ± 3.4% was seen in the SCCVII tumours. The highest contrast medium uptake of 10.1 ± 1.1% was noted in the M21 tumours, and 14.8 ± 1.9% in the SCCVII tumours. In all tumours, a significant increase in the diffusion coefficient was seen with increased HIFU intensity, the highest of which was 40.3 ± 4.1% in the SCCVII tumours. The three tumour cell lines stably transfected with the hsp70/luciferase gene showed differential luciferase activity, which peaked at different times after the application of HIFU and was dependant on tumour type and HIFU energy deposition. |
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Authors:
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W Hundt; S Steinbach; M Burbelko; A Kiessling; M Rominger; C E O'Connell-Rodwell; D Mayer; M D Bednarski; S Guccione |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Technology in cancer research & treatment Volume: 10 ISSN: 1533-0338 ISO Abbreviation: Technol. Cancer Res. Treat. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101140941 Medline TA: Technol Cancer Res Treat Country: United States |
Other Details:
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Languages: eng Pagination: 197-210 Citation Subset: IM |
Affiliation:
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Department of Radiology, Lucas MRS Research Center, Stanford School of Medicine, Stanford, CA USA. hundt@med.uni-marburg.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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